Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Phosphorylated RNAPII interactome via 4H8 antibody IP

ABSTRACT: 4H8 pull down of chromatin-associated proteins in the Flp-In T-REx HEK293 parental cell line. Phosphorylated RNAPII interactome was measured in three conditions; Untreated, 1mM H2O2 treatment for 15minutes, and 1 hour recovery

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Michael Wierer

LAB HEAD: Lea H. Gregersen

PROVIDER: PXD067969 | Pride | 2026-06-22

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	experimental_design.txt	Txt
	mqpar.xml	Xml
	raw.zip	Other
	txt.zip	Other

Items per page:

1 - 4 of 4

Similar Datasets

Phosphorylated RNAPII interactome via 4H8 antibody IP with OGG1 inhibitor

Project description:RNAPII pull down of chromatin associated proteins in the Flp-In T-REx HEK293 parental cell line. Phosphorylated RNAPII interactome was measured in six conditions; Untreated (with and without OGG1i), 1mM H2O2 treatment for 15minutes (with and without OGG1i) and 2 hours recovery following media washout (with and without OGG1i)

2026-06-22 | PXD070086 | Pride

RNAPII interactome via RPB3-FLAG IP

Project description:FLAG Ab pull down of chromatin associated proteins in the stable cell line expressing RPB3-3xFLAG (with DOX). Total RNAPII interactome was measured in three conditions; Untreated, 1mM H2O2 treatment for 15 minutes, and 1 hour recovery following media washout.

2026-06-22 | PXD067831 | Pride

RNAPII interactome in INTS6 degron cells

Project description:In this study we show that acute INTS6 depletion remodels the RNAPII interactome and enhances ARMC5 binding, the same as what we observed in Ints6 KO cells. To investigate how acute INTS6 depletion affects RNAPII function, we determined the RNAPII interactome in the the Ints6 degron cells. Chromatin fractions from DMSO and dTAG treated Ints6 degron cells (treated for 1 hour) were analyzed by RNAPII IP-mass spectrometry. There are 12 samples in this MS run, samples 6-dTAG repeats1-3 and 6-DMSO repeats 1-3 are involved in the paper.

2025-12-22 | PXD053351 | Pride

RNAPII interactome in INTS6 KO and WT cells

Project description:In this study we show that INTS6 KO remodels the RNAPII interactome and enhances ARMC5 binding. To investigate how INTS6 affects RNAPII function, we determined the RNAPII interactome in the presence and absence of INTS6 using Ints6 KO cells. Chromatin fractions from WT and INTS6 KO cells, with or without DRB treatment, were analyzed by RNAPII IP-mass spectrometry.

2025-12-22 | PXD053346 | Pride

Whole cell proteome of cells lacking heme biosynthesis enzyme CPOX

Project description:Whole cell proteomics of a CRISPR/Cas9-generated HEK293 FLP/IN/T-Rex CPOX KO cell line in comparison to HEK293 FLP/IN/T-Rex WT cells and HEK293 FLP/IN/T-Rex CPOX KO cells complemented with CPOX-HA WT.

2026-04-16 | PXD064668 | Pride

PP1-NIPP1 wild type and mutant overexpression in Flip-In™ T-Rex Hela cells

Project description:RNAi-seq of stable Flp-In™ T-Rex Hela cells that inducibly expressed PP1-NIPP1 and mutants.

2018-06-28 | GSE104320 | GEO

Effects of pan-UPF1 knockdown on RNA abundance

Project description:Flp-In T-REx-293 cells transfected with non-targeting control siRNA or UPF1-specific siRNA as indicated and used for total RNA-seq.

2022-04-07 | GSE176197 | GEO

Anti-FLAG co-immunoprecipitation of N- or C-terminally FLAG-HA-tagged FAM98A, FAM98B and FAM98C, from HEK Flp-In T-REx 293 cells.

Project description:Determination of the interactome of different human FLAG-HA-tagged FAM98 paralogs (FAM98A, FAM98B and FAM98C) by co-immunoprecipitation/MS. Two expression constructs were used per paralog: one with an N-terminal and one with a C-terminal FLAG-HA-tag. Expression constructs were stably integrated into HEK Flp-In T-REx 293 cells and induced by addition of doxycycline. HEK Flp-In T-REx 293 cells without any integrated construct were used as control.

2026-06-08 | PXD076703 | Pride

Uridilation by TUT4/7 restricts retrotransposition of human Line-1s

Project description:Purpose: the goal of this study was to test whether the amounts of genome-encoded Line-1s are influenced by TUTases and Mov10 Methods: RNA-Seq data were obtained for PA-1 or Hek293 Flp-IN T-Rex cells in which wild-type or mutant TUTases or Mov10 were overexpressed or the proteins were depleted by RNA interference Results: Minor changes (less than 0.4-fold) were observed in the amounts of mRNAs of Homo sapiens-specific Line-1 families in Hek293 Flp-IN T-Rex and PA-1 either overexpressing or depleted of TUTases and Mov10

2018-08-15 | GSE105264 | GEO

Mass spectrometry analyses of Strep-PCID2/GANP immunoprecipitations

Project description:Mass spectrometry analyses of Strep-PCID2/GANP immunoprecipitations prepared from Flp-In T-REx 293 cells

| PXD068408 |