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Proteomics on pancreatic ductal organoids

ABSTRACT: Primary human pancreatic ductal organoids (HPDO) have emerged as a model to study pancreas biology and model disease like pancreatitis and pancreatic cancer. Yet, donor material availability, genetic variability and a lack of extensive benchmarking to healthy and disease pancreas limits the range of applications. To address this gap, we established porcine pancreatic ductal organoids (PPDO) as a system from a reliable, genetically defined and easily obtainable source to model pancreatic ductal/progenitor biology. We benchmarked PPDO to HPDO and primary porcine pancreas using single-cell RNA sequencing (scRNA-Seq). We observed no overt phenotypic differences in PPDO derived from distinct developmental stages using extensive proteomics profiling, with a WNT/basal cell signaling enriched population characterizing PPDO. PPDO exhibited differentiation potential towards mature ductal cells and limited potential towards endocrine lineages. We used PPDO as a chemical screening platform to assess the safety of FDA-approved drugs and showed conserved toxicity of statins and α-adrenergic receptor inhibitors between PPDO and HPDO cultures. Overall, our results highlight the PPDO as a model for mammalian duct/progenitor applications.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Pancreas

SUBMITTER: Christine von Toerne

LAB HEAD: Heiko Lickert

PROVIDER: PXD068063 | Pride | 2025-11-10

REPOSITORIES: Pride

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			Action	DRS
	KAC116_X002_H2528_tissue_WH.raw	Raw
	KAC116_X003_H2535_tissue.raw	Raw
	KAC116_X004_H2557_tissue.raw	Raw
	KAC116_X005_H2559_tissue.raw	Raw
	KAC116_X007_H2494_organoids.raw	Raw

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