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Proteomic analysis of hBM-MSCs and hBM-MSC-derived EVs following lipid perturbations with exogenous sphingomyelinase

ABSTRACT: This experiment describes the protomic profiling of human bone marrow-dervied Mesenchymal Stromal Cells and their derived extracellular vesicles following an exogenous treatment of MSCs with neutral sphingomyelinase. Samples were ran in triplicate biological replicates with 1 MSC donor. EV samples were processed using TMTpro 16 plex labeling kit. Samples were injected and measured using a Q exactive Plus high-reslution tandem mass spectrometer.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Daniel Shah

LAB HEAD: Edward Botchwey

PROVIDER: PXD068127 | Pride | 2025-09-05

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20240607_DS_EV_TMT_R1.raw	Raw
	20240607_DS_EV_TMT_R1R2.msf	Msf
	20240607_DS_EV_TMT_R2.raw	Raw
	20240610_DS_CellLysateLFQ.msf	Msf
	20240610_DS_cS1.raw	Raw

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