Proteomics

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Effect of 3’,4’-dihydroxyflavonol eye drops in a rat model of dispase-induced proliferative vitreoretinopathy

ABSTRACT: Proliferative vitreoretinopathy (PVR) was induced in a rat model using intravitreal dispase to study fibrosis- and inflammation-related proteome changes and to evaluate the therapeutic effect of topical 3’,4’-dihydroxyflavonol (DiOHF) eye drops. Retina and vitreous were collected 28 days post-injury from vehicle-, DiOHF- and methotrexate-treated eyes, alongside contralateral controls. In parallel, ARPE-19 cells were stimulated with TGFβ1 + PDGF-BB to model wound-healing responses in vitro, with or without DiOHF. Label-free DIA LC-MS/MS was performed (Orbitrap Exploris 480 for rat tissues; Orbitrap Eclipse for ARPE-19), and data were analyzed with Spectronaut directDIA against UniProt Rattus norvegicus or Homo sapiens canonical databases. The dataset contains the original instrument raw files and processed Spectronaut results for both the rat retina/vitreous and ARPE-19 experiments.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Retinal Pigment Epithelial Cell, Retina

DISEASE(S): Glaucoma

SUBMITTER: cheng zeng  

LAB HEAD: Cheng Zeng

PROVIDER: PXD068151 | Pride | 2026-01-05

REPOSITORIES: Pride

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241018_EC-9611_01.raw Raw
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241018_EC-9611_03.raw Raw
241018_EC-9611_04.raw Raw
241018_EC-9611_05.raw Raw
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Publications

Effect of 3',4'-Dihydroxyflavonol Eye Drops in a Rat Model of Dispase-Induced Proliferative Vitreoretinopathy.

Chan Elsa C EC   Zeng Cheng C   Luu Chi D CD   Abbott Carla J CJ   Chan Nicholas T NT   Datta Keshava K KK   Williamson Nicholas N   Allen Penelope J PJ   Fan Gaskin Jennifer C JC  

Antioxidants (Basel, Switzerland) 20251127 12

(1) Background: Proliferative vitreoretinopathy (PVR) is the most common cause of failure in retinal detachment surgery and often leads to blindness. Oxidative stress is known to contribute to scar formation; therefore, reducing oxidative stress may protect against PVR development. This study investigated the therapeutic effects of the antioxidant 3',4'-dihydroxyflavonol (DiOHF) in two preclinical models of PVR. (2) Methods: A retinal pigment epithelial cell line (ARPE-19) was used to investigat  ...[more]

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