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Mass spectrometry proteomics data for the Spy protein from Escherichia coli WT and ∆msrP during chlorite stress.

ABSTRACT: Shotgun proteomic analysis of the 15 kDa proteome of WT and ∆msrP Escherichia coli cells during chlorite stress focussed on the Spy protein

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean ARMENGAUD

PROVIDER: PXD068189 | Pride | 2025-09-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	E24793_Pres-036_chro_wt-15kDa_tube1.mgf	Mgf
	E24793_Pres-036_chro_wt-15kDa_tube1.raw	Raw
	E24795_Pres-036_chro_DmsrP-15kDa_tube3.mgf	Mgf
	E24795_Pres-036_chro_DmsrP-15kDa_tube3.raw	Raw
	F236436.dat	Other

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Project description:Escherichia coli spheroplast protein y (EcSpy) is a small periplasmic protein that is homologous with CpxP, an inhibitor of the extracytoplasmic stress response. Stress conditions such as spheroplast formation induce the expression of Spy via the Cpx or the Bae two-component systems in E. coli, though the function of Spy is unknown. Here, we report the crystal structure of EcSpy, which reveals a long kinked hairpin-like structure of four ?-helices that form an antiparallel dimer. The dimer contains a curved oval shape with a highly positively charged concave surface that may function as a ligand binding site. Sequence analysis reveals that Spy is highly conserved over the Enterobacteriaceae family. Notably, three conserved regions that contain identical residues and two LTxxQ motifs are placed at the horizontal end of the dimer structure, stabilizing the overall fold. CpxP also contains the conserved sequence motifs and has a predicted secondary structure similar to Spy, suggesting that Spy and CpxP likely share the same fold.

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Project description:Dictyostelium discoideum O-Thr-fucosylated proteins from WT and SPY-KO vegetative cells were immunoprecipitated using anti O-Thr fucose polyclonal antibodies in 3 Biological Replicates, with 3 to 6 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).

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