Proteomics

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Melatonin Rescues Triclosan-Disrupted Porcine Oocyte Meiosis via Suppression of p53-Mediated Apoptosis

ABSTRACT: This study investigates the toxic effects of the environmental contaminant triclosan (TCS) on porcine oocyte meiosis and the protective role of melatonin (MT). Using an in vitro maturation model, TCS was found to impair polar body extrusion and cumulus expansion, while inducing oxidative stress, mitochondrial and endoplasmic reticulum dysfunction, and apoptosis. To explore the underlying molecular mechanisms, we performed proteomic profiling using a Bruker timsTOF Pro2 mass spectrometer with a Direct DIA acquisition strategy. Differentially expressed proteins (DEPs) were quantified and analyzed using Spectronaut software, followed by functional annotation and enrichment analysis via GO, KEGG, and Reactome databases. The results revealed that the p53 signaling pathway plays a central role in both TCS-induced toxicity and MT-mediated rescue, providing new insights into reproductive damage caused by environmental pollutants.

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa (pig)

TISSUE(S): Oocyte

SUBMITTER: Ning Zhao  

LAB HEAD: Guoqing Cao

PROVIDER: PXD068303 | Pride | 2025-11-17

REPOSITORIES: Pride

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0-1_slot1-1_1_5104.d.zip Other
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0-3_slot1-1_1_5106.d.zip Other
20240806_140010_GDP23111103-2.sne Other
TCS-1_slot1-1_1_5108.d.zip Other
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Melatonin Rescues Triclosan-Disrupted Porcine Oocyte Meiosis via Suppression of p53-Mediated Apoptosis.

Duan Jiaxin J   Zhao Ning N   Wang Shibin S   Li Xinyu X   Li Bugao B   Cao Guoqing G  

Animals : an open access journal from MDPI 20251103 21

Triclosan (TCS), a widely used environmental antimicrobial agent, poses potential risks to female reproductive health, yet its toxic effects on oocyte maturation remain inadequately characterized. In this study, we established an in vitro maturation (IVM) model of porcine oocytes to investigate TCS-induced meiotic impairment and to evaluate the rescuing effects of melatonin (MT), an endogenous indoleamine with potent antioxidant and anti-apoptotic activities. Our results demonstrated that TCS ex  ...[more]

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