Proteomics

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Mistranslating tRNA variants impact on the proteome and phosphoproteome of Saccharomyces cerevisiae – phosphoproteome DDA and DIA data

ABSTRACT: Mutations in tRNAs can lead to mis-incorporation of an amino acid into a growing polypeptide chain that differs from what is specified by the mRNA in a process known as mistranslation. As mistranslating tRNAs modify how the genetic message is decoded, they have potential as therapeutic tools to treat diseases caused by nonsense and missense mutations. However, they also produce proteome-wide mis-made proteins which can disrupt proteostasis. To better understand the impact of mistranslating tRNA variants, we profile the proteome and phosphoproteome of yeast expressing three different mistranslating tRNAs. The first is a proline tRNA with a G3:U70 base pair in its acceptor stem that mis-incorporates alanine at proline codons (Pro→Ala; Hoffman et al. 2017). The other two are serine tRNAs with either a UGG proline or UCU arginine anticodon which mis-incorporate serine at proline (Pro→Ser) or serine at arginine codons (Arg→Ser), respectively (Berg et al. 2017, 2019). This submission includes 24 DIA-MS raw files and 24 DDA-MS raw files from phospho-enriched samples.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Matthew Berg  

LAB HEAD: Judit Villén

PROVIDER: PXD068388 | Pride | 2025-11-18

REPOSITORIES: Pride

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Dataset's files

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Action DRS
FragPipe_Phosphoproteome_library.tsv Tabular
Phosphoproteome_DIANNreport.parquet Other
Phosphoproteome_RawRunAnnotations.csv Csv
checksum.txt Txt
e14486_MB057_Ctrl_01_Phospho_DIA.mzML Mzml
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Publications

Mistranslating tRNA variants impact the proteome and phosphoproteome of <i>Saccharomyces cerevisiae</i>.

Berg Matthew D MD   Chang Alexis T AT   Rodriguez-Mias Ricard A RA   Villén Judit J  

bioRxiv : the preprint server for biology 20250918

Transfer RNAs (tRNAs) ensure accurate decoding of the genetic code. However, mutations in tRNAs can lead to mis-incorporation of an amino acid that differs from the genetic message in a process known as mistranslation. As mistranslating tRNAs modify how the genetic message is decoded, they have potential as therapeutic tools for diseases caused by nonsense and missense mutations. Despite this, they also produce proteome-wide mis-made proteins which can disrupt proteostasis. To better understand  ...[more]

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