Proteomics

Dataset Information

Mass spectrometry-based validation of BPTF knockout in human induced pluripotent stem cells 

ABSTRACT: We generated a BPTF knockout human induced pluripotent stem cell (iPSC) line using CRISPR-Cas9 and confirmed loss of the targeted locus by genotyping. Quantitative mass spectrometry verified complete absence of BPTF protein in the knockout clone. This validated line was used for downstream functional assays in the somitoid system, a 3D iPSC-derived model that recapitulates early human somite development.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Hipsc Cell

SUBMITTER: S Stelloo  

LAB HEAD: Michiel vermeulen

PROVIDER: PXD068407 | Pride | 2026-08-07

REPOSITORIES: Pride

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20250910_Astral2_AUR_MA_30SPD_WCE_SP3_hiPSCs_BPTF_KO_1.raw Raw
20250910_Astral2_AUR_MA_30SPD_WCE_SP3_hiPSCs_BPTF_KO_2.raw Raw
20250910_Astral2_AUR_MA_30SPD_WCE_SP3_hiPSCs_BPTF_KO_3.raw Raw
20250910_Astral2_AUR_MA_30SPD_WCE_SP3_hiPSCs_WT_1.raw Raw
20250910_Astral2_AUR_MA_30SPD_WCE_SP3_hiPSCs_WT_2.raw Raw
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