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Characterisation of corneal defects in ATG7-deficient mice

ABSTRACT: Proteomic analysis of mouse cornea lysates. ATG7 is an autophagy component necessary for thedegradation and recycling of cellular components. We aim to characterise the difference in corneal phenotype between an ATG7 knockdown mouse model and a control mouse model.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cornea

SUBMITTER: Prerana Wagle

LAB HEAD: Maria Notara

PROVIDER: PXD068453 | Pride | 2025-11-10

REPOSITORIES: Pride

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			Action	DRS
	3357_lib_MBR.tsv.speclib	Tabular
	E3_3357_01.raw	Raw
	E3_3357_02.raw	Raw
	E3_3357_03.raw	Raw
	E3_3357_04.raw	Raw

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Publications

Characterization of Corneal Defects in ATG7-Deficient Mice.

Volatier Thomas T Mourier Andreas A Mann Johanna J Meshko Berbang B Hadrian Karina K Cursiefen Claus C Notara Maria M

International journal of molecular sciences 20251014 20

Regulated proteolysis via autophagy is essential for cellular homeostasis, yet the specific role of autophagy-related gene 7 (ATG7) in corneal epithelial maintenance remains unclear. Using a conditional knockout mouse model (Atg7f/f K14Cre+/-), we investigated the impact of ATG7 deficiency on corneal epithelial autophagy, morphology, and vascular dynamics. Loss of ATG7 disrupted autophagosome formation, evidenced by increased LC3B expression but reduced LC3B-positive ...[more]

PMID: 41155280

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