Dataset Information

Proteomic profiling of Levilactobacillus brevis CRL 2013 grown in different nitrogen sources.

ABSTRACT: This project aimed to characterize the proteomic profile of Levilactobacillus brevis CRL 2013, a γ-aminobutyric acid (GABA)-producing lactic acid bacterium, grown under different nitrogen sources (CDMg, CDMgC, CDMgYE). Proteins were extracted from cultures, digested with trypsin, and analyzed using nanoLC coupled to high-resolution tandem mass spectrometry on a Q-Exactive Orbitrap instrument. The study provides insights into protein expression changes linked to metabolic adaptation under varying nutritional conditions. Raw MS data and search engine results are deposited to enable re-analysis and comparison with related studies.

INSTRUMENT(S):

ORGANISM(S): Levilactobacillus Brevis Atcc 367

SUBMITTER: Elvira Hebert

LAB HEAD: Elvira Maria Hebert

PROVIDER: PXD068495 | Pride | 2026-01-19

REPOSITORIES: Pride

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Publications

Influence of Peptide-Rich Nitrogen Sources on GAD System Activation and GABA Production in <i>Levilactobacillus brevis</i> CRL 2013.

Urquiza Martínez María Paulina MP Cataldo Pablo G PG Ríos Colombo Natalia Soledad NS Ferranti Pasquale P Saavedra Lucila L Hebert Elvira M EM

International journal of molecular sciences 20251221 1

γ-Aminobutyric acid (GABA) is a bioactive metabolite valued in functional foods, but its microbial production is strongly influenced by nutrient availability. <i>Levilactobacillus brevis</i> CRL 2013 is an efficient GABA producer; however, its biosynthesis depends on culture medium composition. In this study, integrated physiological, proteomic, and transcriptional analyses were applied to assess the influence of nitrogen source composition and concentration on GABA production. No extracellular ...[more]

PMID: 41515963

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Project description:Background: Of the many neurotransmitters in humans, gamma-aminobutyric acid (GABA) shows potential for improving several mental health indications such as stress and anxiety. The microbiota-gut-brain axis is an important pathway for GABAergic effects, as microbially-secreted GABA within the gut can affect host mental functionhealth outcomes. Understanding the molecular characteristics of GABA production by microbes within the gut can offer insight to novel therapies for mental health. Results: Three strains of Levilactobacillus brevis with syntenous glutamate decarboxylase (GAD) operons were evaluated for overall growth, glutamate utilization, and GABA production in typical synthetic growth media supplemented with monosodium glutamate (MSG). Levilactobacillus brevis Lbr-6108 (Lbr-6108) and Levilactobacillus brevis Lbr-35 (Lbr-35) had similar growth profiles but differed significantly in GABA secretion and acid resistance. Lbr-6108 produced GABA early, within the growth phase, and produced significantly more GABA than Lbr-35 and the type strain Levilactobacillus brevis ATCC 14689 after the stationary phase. The global gene expression during GABA production was determined by RNA sequencing at several timepoints. The GAD operon, responsible for GABA production and secretion, activated in Lbr-6108 after only six hours of fermentation and continued throughout the stationary phase. Furthermore, Lbr-6108 activated many different acid resistance mechanisms concurrently, which contribute to acid tolerance and energy production. In contrast, Lbr-35, which has a genetically similar GAD operon, including two copies of the GAD gene, showed no upregulation of the GAD operon, even when cultured with MSG. Conclusions: This study is the first to evaluate whole transcriptome changes in L. brevis during GABA production over multiple timepoints. The concurrent expression of multiple acid-resistance mechanisms reveals niche-specific metabolic functionality between common human commensals and highlights the complex regulation of GABA metabolism in this important microbial species. Furthermore, the increased and rapid GABA production of Lbr-6108 highlights the strain’s potential as a therapeutic and the overall value of screening microbes for effector molecule output.

			Action	DRS
	PC01.pdResult	Other
	PC01.raw	Raw
	PC01alPC09.sps	Other
	PC02.pdResult	Other
	PC02.raw	Raw

Dataset Information

Proteomic profiling of Levilactobacillus brevis CRL 2013 grown in different nitrogen sources.

Dataset's files

Publications

Influence of Peptide-Rich Nitrogen Sources on GAD System Activation and GABA Production in <i>Levilactobacillus brevis</i> CRL 2013.

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Dataset Information

Proteomic profiling of Levilactobacillus brevis CRL 2013 grown in different nitrogen sources.

Dataset's files

Publications

Influence of Peptide-Rich Nitrogen Sources on GAD System Activation and GABA Production in &lt;i&gt;Levilactobacillus brevis&lt;/i&gt; CRL 2013.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Influence of Peptide-Rich Nitrogen Sources on GAD System Activation and GABA Production in <i>Levilactobacillus brevis</i> CRL 2013.