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Supplementary Dataset S4. Comparison of proteins identified in three experiments using the functional (Af1521) versus non-functional (G42E) Macro domains following ADPr-enrichment of proteins from Dex-treated AvrRpm1-HA plants.

ABSTRACT: During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to compare affinity enrichment of ADP-ribosylated Arabidopsis thaliana proteins using the enhanced Af1521 Macro domain versus a G42E mutant variant as control.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Lennart Wirthmueller

LAB HEAD: Lennart Wirthmueller

PROVIDER: PXD068551 | Pride | 2025-11-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ADP-ribosylationSites.txt	Txt
	AvrRpm1_Af1521_exp1.raw	Raw
	AvrRpm1_Af1521_exp2.raw	Raw
	AvrRpm1_Af1521_exp3.raw	Raw
	AvrRpm1_G42E_exp1.raw	Raw

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Supplementary Datasets S2 and S3. ADP-ribosylated peptides identified in three Af1521 Macro pulldown experiments from AvrRpm1-HA samples by the standard method (S2) and the 'triggering' method (S3).

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Supplementary Dataset S7. ADP-ribosylated peptides identified in two Af1521 Macro pulldown experiments from HopU1-Flag vs. Col-0 samples by the ‘triggering’ method.

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Supplementary Dataset S5. AvrRpm1 ADP-ribosylates MAMI when expressed in N. benthamiana.

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Supplementary Dataset S6. AvrRpm1 ADP-ribosylates PLDGAMMA3 when expressed in N. benthamiana.

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