Proteomics

Dataset Information

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Proteomic validation of saprophyticin S (SapS), a novel leaderless bacteriocin from Staphylococcus saprophyticus P1CEA4

ABSTRACT: This project reports the identification and characterization of saprophyticin S (SapS), a novel leaderless bacteriocin produced by Staphylococcus saprophyticus P1CEA4, a strain isolated from the porcine gastrointestinal tract. MALDI-TOF MS analysis revealed SapS variants corresponding to formylated and oxidized isoforms. To validate peptide identity, the active FPLC fraction was digested with trypsin and analyzed by LC-MS/MS. Proteome Discoverer 2.4 (Mascot search) initially provided 16% sequence coverage, while PEAKS Studio 10.5 improved coverage to 78%, identifying multiple peptides consistent with the predicted SapS sequence. These results provide strong proteomic evidence for SapS production in the native strain and support subsequent functional and structural characterization. The dataset contains processed outputs (Excel and HTML files) from Proteome Discoverer and PEAKS analysis, including peptide identifications, scores, probabilities, PTMs, and coverage statistics.

INSTRUMENT(S):

ORGANISM(S): Staphylococcus Saprophyticus

SUBMITTER: Concha Gil  

LAB HEAD: Juan Borrero del Pino

PROVIDER: PXD068573 | Pride | 2026-06-08

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
24-16_P1CEA4-10.raw Raw
24-16_P1CEA4-10_newsample_ALL-2.4.0.305.mgf Mgf
24-16_P1CEA4-10_newsample_ALL-2.4.0.305.mzML Mzml
24-16_P1CEA4-10_newsample_ALL-2.4.0.305.mzid Mzid
24-16_P1CEA4-10_newsample_ALL.raw Raw
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Publications

Saprophyticin S: A Novel Saposin-like, Leaderless Bacteriocin Produced by Staphylococcus saprophyticus P1CEA4 of Porcine Origin.

Sevillano Ester E   Lafuente Irene I   Peña Nuria N   Cintas Luis M LM   Muñoz-Atienza Estefanía E   Hernández Pablo E PE   Borrero Juan J  

Probiotics and antimicrobial proteins 20251103 3

With antimicrobial resistance (AMR) posing a major global threat, largely driven by antibiotic overuse, the search for alternative antimicrobial strategies has become increasingly urgent. Bacteriocins, due to their specificity and limited impact on host microbiota, are gaining attention as viable substitutes. Here, we report the identification and characterization of a novel bacteriocin produced by Staphylococcus saprophyticus P1CEA4, a strain isolated from meat-producing pigs. This strain exhib  ...[more]

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