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LIC phosphorylated by PI4Kγ7, LC-MS/MS

ABSTRACT: High yield and wide adaptation of rice is of significance for more grain supply and food security. Here, we report a type II phosphatidylinositol 4-kinase, PI4Kγ7, harboring a PI3/PI4 kinase domain, which positively regulates rice yield and affords wide adaptation of rice. We show that PI4Kγ7 interacts with a CCCH-type zinc finger transcription factor, OsLIC. Further investigations discover that PI4Kγ7 modulates rice yield by facilitating the stability and cytoplasm-to-nucleus translocation of OsLIC through phosphorylation. To identify the residue(s) being phosphorylated by PI4Kγ7, we performed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis

INSTRUMENT(S):

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Rui Zhu

LAB HEAD: MS Rui Zhu

PROVIDER: PXD068616 | Pride | 2025-12-08

REPOSITORIES: Pride

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			Action	DRS
	LIC.txt	Txt
	checksum.txt	Txt
	zhurui.zip	Other

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Project description:Enhancing grain production of rice (Oryza sativa L.) is a top priority in ensuring food security for human being. One approach to increase yield is to delay leaf senescence and to extend the available time for photosynthesis. microRNAs (miRNAs) are key regulators for aging and cellular senescence in eukayotes. However, miRNAs and their roles in rice leaf senescence remain unexplored. Here, we report identification of miRNAs and their putative target genes by deep sequencing of six small RNA libraries, six RNA-seq libraries and two degradome libraries from the leaves of two super hybrid rice, Nei-2-You 6 (N2Y6, age-resistant rice) and Liang-You-Pei 9 (LYP9, age-sensitive rice). Totally 372 known miRNAs and 162 miRNA candidates were identified, and 1145 targets were identified. Compared with the expression of miRNAs in the leaves of LYP9, the numbers of miRNAs up-regulated and down-regulated in the leaves of N2Y6 were 47 and 30 at early stage of grain-filling, 21 and 17 at the middle stage, and 11 and 37 at the late stage, respectively. Six miRNA families, osa-miR159, osa-miR160 osa-miR164, osa-miR167, osa-miR172 and osa-miR1848, targeting the genes encoding APETALA2 (AP2), zinc finger proteins, salicylic acid-induced protein 19 (SIP19), Auxin response factors (ARF) and NAC transcription factors, respectively, were found to be involved in leaf senescence through phytohormone signaling pathways. These results provided valuable information for understanding the miRNA-mediated leaf senescence of rice, and offered an important foundation for rice breeding. [miRNA] sample 1:The flag leaves at early stage of grain-filling of N2Y6 rice; sample 2: The flag leaves at middle stage of grain-filling of N2Y6 rice;sample 3:The flag leaves at late stage of grain-filling of N2Y6 rice; sample 4:The flag leaves at early stage of grain-filling of LYP9 rice; sample 5: The flag leaves at middle stage of grain-filling of LYP9 rice;sample 6:The flag leaves at late stage of grain-filling of LYP9 rice. [DGE]: samples 7-12 [degradome (targets)]: samples 13:The flag leaves at mixed stages of grain-filling of N2Y6 rice; sample 14:The flag leaves at mixed stages of grain-filling of LYP9 rice

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LIC phosphorylated by PI4Kγ7, LC-MS/MS

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