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Proteome Analyses of Human NK Cells after alloSCT Challenged byAspergillus fumigatus

ABSTRACT: We collected longitudinally human NK cells from patients after alloSCT (at days +90, +120, +180), to identify any potential functional NK cell defects on a protein level. We have focused our analysis on whole proteome patterns by analyzing patient NK cells stimulated with A. fumigatus or left unstimulated, in comparison to those obtained from healthy controls. In parallel, a broad collection of clinical data from these patients was archived. This data revealed specific, quite different protein patterns, depending on the status of the NK cells. We were able to define significant differences in the proteome composition of NK cell isolated from alloSCT recipients compared to healthy controls. The majority of these differences were present until day +180. Fungal stimulation of NK cells led initially to markedly differential proteomes while at day +180 only a small number of proteins were differentially expressed between A. fumigatus stimulated NK cells from alloSCT recipients compared to healthy controls. Furthermore, we were able to confirm our proteome data by flow cytometry.

INSTRUMENT(S):

ORGANISM(S): Neosartorya Fumigata (aspergillus Fumigatus) Homo Sapiens (human)

SUBMITTER: Thomas Krüger

LAB HEAD: Axel A. Brakhage

PROVIDER: PXD068702 | Pride | 2026-04-30

REPOSITORIES: Pride

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