Dataset Information

E. coli slow recovery from growth arrest

ABSTRACT: Bacteria shut down protein synthesis under stress, but how they resume growth remains unclear. We used an E. coli strain lacking a ribosome maturation factor RimM, which exhibits prolonged growth arrest, as a model to study bacterial recovery. Loss of this factor delays 30S ribosomal subunit assembly more than other maturation factors such as RbfA. Mass spectrometry and cryo-EM analyses revealed disrupted assembly of the 30S head domain, increased binding of initiation factors (IF1 and IF3), and recruitment of a silencing factor RsfS to the 50S subunit, collectively preventing premature 70S formation.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli Bacteria

TISSUE(S): Cell Culture

SUBMITTER: Pavel Roudnický

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD068732 | Pride | 2026-03-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	6705_HT1_108m_DIA_1_1_BA7_1_8766.d.zip	Other
	6705_HT1_108m_DIA_2_1_BA8_1_8774.d.zip	Other
	6705_HT1_108m_DIA_3_1_BB6_1_8782.d.zip	Other
	6705_HT1_108m_DIA_4_1_BB7_1_8726.d.zip	Other
	6705_HT1_108m_DIA_5_1_BB8_1_8814.d.zip	Other

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Publications

Mechanistic insights into E. coli recovery from growth arrest.

Hassan Ahmed H AH Nakano Yuko Y Gamper Howard H Masuda Isao I Vesela Ludmila L Pinkas Matyas M Nagarajan Sathya Narayanan SN Dworkin Jonathan J Blaha Gregor G Hou Ya-Ming YM Demo Gabriel G

Nature communications 20260411

Bacteria survive hostile conditions by shutting down protein synthesis, but how they restart growth remains poorly understood. Here, we use an E. coli ΔrimM strain, which exhibits a prolonged growth arrest, as a model to investigate how bacteria recover from this state and restore protein synthesis. RimM is a conserved ribosome maturation factor for the 3'-major (head) domain of the 16S rRNA within the bacterial 30S subunit. The loss of RimM causes a longer delay in recovery than other 30S matur ...[more]

PMID: 41965359

Dataset Information

E. coli slow recovery from growth arrest

Dataset's files

Publications

Mechanistic insights into E. coli recovery from growth arrest.

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