Dataset Information

A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: Qual-Quan Data Sets for PAMAF and DDA Modes

ABSTRACT: Here we present a parallel accumulation-mobility aligned fragmentation (PAMAF) mode that expands the capabilities of the MOBILion® Systems MOBIE® high-resolution ion mobility (HRIM) system for mass spectrometry-based proteomics. The MOBIE system leverages structures for lossless ion manipulation (SLIM) to achieve HRIM separations within a compact footprint. Along with the HRIM separations, which increase the number of features identified per cycle, PAMAF enables alignment of fragment ions with their precursors ions without quadrupole isolation prior to fragmentation, avoiding the ion losses up to 99%. With PAMAF mode, ions can be accumulated in parallel to the ion mobility (IM) separation, achieving close to 100% ion utilization efficiency. Quantitative evaluations demonstrated that PAMAF mode was capable of quantifying low abundance peptides, including those undetectable by DDA.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Leonard Rorrer

LAB HEAD: Daniel DeBord

PROVIDER: PXD068773 | Pride | 2026-07-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	100fmolQQ_200SPD_r001.d.zip	Other
	100fmolQQ_200SPD_r001.mzML	Mzml
	100fmolQQ_200SPD_r002.d.zip	Other
	100fmolQQ_200SPD_r002.mzML	Mzml
	100fmolQQ_200SPD_r003.d.zip	Other

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Publications

A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis.

Rorrer Leonard C LC Deng Liulin L Royer Lauren L Uribe Isabel I Orsburn Benjamin C BC Bernhardt Oliver O Gandhi Tejas T Reiter Lukas L DeBord Daniel D

Molecular & cellular proteomics : MCP 20260622

Here we present a novel data independent acquisition (DIA) mass spectrometry (MS) operating mode termed parallel accumulation-mobility aligned fragmentation (PAMAF) that offers enhanced speed and sensitivity of ion fragmentation analysis for discovery workflows such as bottom-up proteomics. This mode of operation leverages high-resolution ion mobility (HRIM) separation capabilities of the structures for lossless ion manipulation (SLIM) technology to achieve HRIM-based precursor isolation in plac ...[more]

PMID: 42331252

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