Dataset Information

Maize anther development involves the function of translated open reading frames from 3’-untranslated regions

ABSTRACT: Currently, few studies have been performed on the function of peptides coding by open reading frames (ORFs) from annotated non-coding regions, especially the 3'-untranslated regions (3'-UTRs). The translated ORFs from 5'-UTRs (5'-ORFs) have been proved that they negatively affect the translation of their main ORFs, but there is little comprehension on 3'-ORFs. This study analyzed the genomic translational regulation events of ten development stages of maize anthers and demonstrated that the peptides translated by 5'- or 3'-ORFs may be misidentified isoforms and the 3'-ORFs may relate to anther sterility, such as the cloned APV1 locus, whose mutation caused male sterility. Furthermore, the global translation events indicated photosynthesis related genes enriched during Phase III, the binucleate microspore phase. The stomata in the anthers and low electron transport rate suggested that they can assimilate CO2 with low photosynthetic efficiency. This study proposed new avenues for the potential creation of male-sterile mutants using 3'-ORFs for hybrid breeding and new evidence of anther photosynthesis.

INSTRUMENT(S):

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Flower

SUBMITTER: M Xuxu

LAB HEAD: Xuxu Ma

PROVIDER: PXD068789 | Pride | 2026-04-27

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 6

Publications

Maize Anther Development Involves Translated Open Reading Frames From 3' Untranslated Regions.

Wang Chunyu C Wu Yuechao Y Wang Shunxi S Liu Han H Jiang Siqi S Han Yingjia Y Wu Liuji L Zhang Tao T Zhang Mei M

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20260220 23

Few studies have explored the functions of peptides encoded by open reading frames (ORFs) in annotated noncoding regions, particularly the 3' untranslated regions (3' UTRs). Although translated ORFs in 5' UTRs (5' ORFs) have been shown to inhibit translation of their corresponding main ORFs (mORFs), the roles of 3' ORFs remain poorly understood. This study analyzes translational regulation across ten developmental stages of maize (Zea mays) anthers and finds that peptides translated from 5' or 3 ...[more]

PMID: 41717821

Similar Datasets

Project description:Chemical hybridization agent (CHA)-induced male sterility is becoming one of the most useful tools for the crop heterosis in seed production. We had previously discovered monosulfuron ester sodium (Mes), an acetolactate synthase (ALS) inhibitor of the herbicide sulfonylurea family, could induce rapeseed (Brassica napus L.) male sterility. To investigate the mechanism of Mes inducing male sterility, a cytological analysis and comparative transcriptome analysis were performed between Mes-treated plants leaves and different developmental stage anthers and those of the Mock-treated plants. Cytological analysis exhibited that Mes destroyed plastids ultrastructure and depressed the materials accumulation during anther development and maturation process. Comparative transcriptome analysis identified a total of 1501 transcripts differentially expressed in the leaves and different developmental stage anthers. Subcellular localization analysis, functional analysis and pathway analysis were carried out to obtain the clues that plastid was the seriously damaged organelle, and many genes involved in carbon and lipid metabolism and cellular transport differentially expressed. Detailed expression pattern analysis of genes related with these functions were conducted and verified by RT-PCR. In addition, several transcription factors, protein kinases and hormone related genes were identified. Carbohydrate content analysis confirmed carbon metabolism were influenced by Mes. Taken together, we proposed a putative action mode that Mes inhibited activity of acetolactate synthase, which localized in plastid in plants, and then disturbed the normal supply of carbon and lipid metabolite for anther development, finally displayed male sterility. This results have important significance for uncovering the metabolic gene regulation during anther development and may provide more potential targets for developing new male sterility inducing CHAs in rapeseed breeding. Rapeseed (Brassica napus L.) plants were foliar sprayed with 0.1Î¼g mL-1 Monosulfuron ester sodium (Mes) solution containing 50ppm DMF and 5ppm Tween 80 for about 15 ml per plant. Young leaves (C2Y1, C2Y2, C2Y3; T2Y1, T2Y2, T2Y3) of the main inflorescences and developing anthers (including small buds (C2S1, C2S2, C2S3; T2S1, T2S2, T2S3), middle anthers (C2M1, C2M2, C2M3; T2M1, T2M2, T2M3), and large anthers (C2L1, C2L2, C2L3; T2L1, T2L2, T2L3)) from Mes-treated and Mock-treated plants were collected for each experiment. Each tissue has three independent biological replicates.

			Action	DRS
	P999-1-15.noredundant	Other
	P999-2-15.noredundant	Other
	P999-3-15.noredundant	Other
	P999-ZMEI-R1.raw	Raw
	P999-ZMEI-R2.raw	Raw