Dataset Information

Coimmunoprecipitation and following mass spectrometry analysis of EhGEFM

ABSTRACT: To find out the binding partners of EhGEFM, crosslinking and coimmunoprecipitation were performed. Then, eluted samples were submitted to Mass Spectrometry and Proteomics Core Facility, Johns Hopkins University School of Medicine and analyzed by mass spctrometry (shotgun proteomics). The coimmunoprecipitation and following analysis were conducted twice. You can find data of the HA-EhGEFM in the first trial from "2024_8_55_44_PM_FileSize_988042288_Byte_F015729.mzid_2024_8_55_44_PM_File_Size__988042288__Byte__F015729.MGF", "2024_8_55_44_PM_FileSize_988042288_Byte_F015729.mzid", and "TB-LI_CS-240315_TsukuiK_KT_1_10p.RAW". You can find data of pEhEx-HA (mock) in the first trial from "2024_12_11_27_AM_FileSize_837709430_Byte_F015730.mzid_2024_12_11_27_AM_File_Size__837709430__Byte__F015730.MGF", "2024_12_11_27_AM_FileSize_837709430_Byte_F015730.mzid", and "TB-LI_CS-240315_TsukuiK_KT_2_10p.RAW". You can find the data of the HA-EhGEFM in the second trial from 2024_2_38_07_PM_FileSize_1529844717_Byte_F016345.mzid_2024_2_38_07_PM_File_Size__1529844717__Byte__F016345.MGF", "2024_2_38_07_PM_FileSize_1529844717_Byte_F016345.mzid", "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF-2.msf", "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF-2.mzML", and "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF.RAW". You can find data of pEhEx-HA (mock) in the second trial from "2024_5_13_15_PM_FileSize_1498194810_Byte_F016346.mzid_2024_5_13_15_PM_File_Size__1498194810__Byte__F016346.MGF", "2024_5_13_15_PM_FileSize_1498194810_Byte_F016346.mzid", "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA-2.msf", "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA-2.mzML", and "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA.RAW"

INSTRUMENT(S):

ORGANISM(S): Entamoeba Histolytica Hm-1:imss

TISSUE(S): Trophozoite

SUBMITTER: Misato Shimoyama

LAB HEAD: Tomoyoshi Nozaki

PROVIDER: PXD068844 | Pride | 2026-05-16

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2024_12_11_27_AM_FileSize_837709430_Byte_F015730.mzid	Mzid
	2024_12_11_27_AM_FileSize_837709430_Byte_F015730.mzid_2024_12_11_27_AM_File_Size__837709430__Byte__F015730.MGF	Mzid
	2024_2_38_07_PM_FileSize_1529844717_Byte_F016345.mzid	Mzid
	2024_2_38_07_PM_FileSize_1529844717_Byte_F016345.mzid_2024_2_38_07_PM_File_Size__1529844717__Byte__F016345.MGF	Mzid
	2024_5_13_15_PM_FileSize_1498194810_Byte_F016346.mzid	Mzid

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Publications

A RhoGEF activates EhRacM to selectively promote motility without affecting macropinocytosis in Entamoeba histolytica.

Shimoyama Misato M Nakada-Tsukui Kumiko K Nozaki Tomoyoshi T

Journal of cell science 20260507

Rho guanine nucleotide-exchange factors (RhoGEFs) activate Rho/Rac small GTPases, orchestrating cellular processes, such as actin remodeling, endocytosis, and migration. In the protozoan parasite Entamoeba histolytica, the causative agent of amebiasis, 22 Rho GTPases and approximately 62 Dbl-homology (DH) domain-containing RhoGEFs have been identified, yet most remain uncharacterized. Our previous work revealed that EhRacM (also known as EhRho13) negatively regulates macropinocytosis while promo ...[more]

PMID: 42093317

Similar Datasets

Project description:To find out the binding partners of EhRacM, crosslinking and coimmunoprecipitation were performed. Then, eluted samples were submitted to Mass Spectrometry and Proteomics Core Facility, Johns Hopkins University School of Medicine and analyzed by mass spctrometry (shotgun proteomics). The coimmunoprecipitation and following analysis were conducted three times.You can find the data of HA-EhRacM (1st) from "2022_7_32_54_PM_FileSize_500998639_Byte_F010303.mzid.gz", "2022_7_32_54_PM_FileSize_500998639_Byte_F010303.mzid_2022_7_32_54_PM_File_Size__500998639__Byte__F010303.MGF", "RO-CS-LE_220912_NozakiT_MS_Rac16_WT.mzML", and "RO-CS-LE_220912_NozakiT_MS_Rac16_WT.RAW". You can find the data of pEhExHA (mock) (1st) from "2022_5_02_01_AM_FileSize_662636563_Byte_F010297.mzid.gz", "2022_5_02_01_AM_FileSize_662636563_Byte_F010297.mzid_2022_5_02_01_AM_File_Size__662636563__Byte__F010297.MGF", "RO-CS-LE_220912_NozakiT_MS_pEhExHA.mzML", and "RO-CS-LE_220912_NozakiT_MS_pEhExHA.RAW".You can find the data of HA-EhRacM (2nd) from "2022_7_35_46_AM_FileSize_794949236_Byte_F009769.mzid.gz", "2022_7_35_46_AM_FileSize_794949236_Byte_F009769.mzid_2022_7_35_46_AM_File_Size__794949236__Byte__F009769.MGF", "JS-QE-CS_221108_NozakiT_MS_S1_RA.msf", "JS-QE-CS_221108_NozakiT_MS_S1_RA.RAW", "JS-QE-CS_221108_NozakiT_MS_S1_RA.mzML". You can find the data of pEhExHA (mock) (2nd) from "2022_2_58_28_AM_FileSize_846222756_Byte_F009771.mzid.gz", "2022_2_58_28_AM_FileSize_846222756_Byte_F009771.mzid_2022_2_58_28_AM_File_Size__846222756__Byte__F009771.MGF", "JS-QE-CS_221108_NozakiT_MS_S4_RA.msf", "JS-QE-CS_221108_NozakiT_MS_S4_RA.RAW", and "JS-QE-CS_221108_NozakiT_MS_S4_RA.mzML". You can find the data of HA-EhRacM (3rd) from "2023_12_15_11_AM_FileSize_1380630557_Byte_F012916.mzid.gz", "2023_12_15_11_AM_FileSize_1380630557_Byte_F012916.mzid_2023_12_15_11_AM_File_Size__1380630557__Byte__F012916.MGF", "JS-E480-CS_230518_NozakiT_MS_S1_DDA_10pct.msf", "JS-E480-CS_230518_NozakiT_MS_S1_DDA_10pct.mzML", and "JS-E480-CS_230518_NozakiT_MS_S1_DDA_10pct.RAW". You can find the data of pEhExHA (mock) (3rd) from "2023_6_35_51_PM_FileSize_1732075728_Byte_F012915.mzid.gz", "2023_6_35_51_PM_FileSize_1732075728_Byte_F012915.mzid_2023_6_35_51_PM_File_Size__1732075728__Byte__F012915.MGF", "JS-E480-CS_230518_NozakiT_MS_S4_DDA_10pct.msf", "JS-E480-CS_230518_NozakiT_MS_S4_DDA_10pct.mzML", and "JS-E480-CS_230518_NozakiT_MS_S4_DDA_10pct.RAW".