Coimmunoprecipitation and following mass spectrometry analysis of EhGEFM

ABSTRACT: To find out the binding partners of EhGEFM, crosslinking and coimmunoprecipitation were performed. Then, eluted samples were submitted to Mass Spectrometry and Proteomics Core Facility, Johns Hopkins University School of Medicine and analyzed by mass spctrometry (shotgun proteomics). The coimmunoprecipitation and following analysis were conducted twice. You can find data of the HA-EhGEFM in the first trial from "2024_8_55_44_PM_FileSize_988042288_Byte_F015729.mzid_2024_8_55_44_PM_File_Size__988042288__Byte__F015729.MGF", "2024_8_55_44_PM_FileSize_988042288_Byte_F015729.mzid", and "TB-LI_CS-240315_TsukuiK_KT_1_10p.RAW". You can find data of pEhEx-HA (mock) in the first trial from "2024_12_11_27_AM_FileSize_837709430_Byte_F015730.mzid_2024_12_11_27_AM_File_Size__837709430__Byte__F015730.MGF", "2024_12_11_27_AM_FileSize_837709430_Byte_F015730.mzid", and "TB-LI_CS-240315_TsukuiK_KT_2_10p.RAW". You can find the data of the HA-EhGEFM in the second trial from 2024_2_38_07_PM_FileSize_1529844717_Byte_F016345.mzid_2024_2_38_07_PM_File_Size__1529844717__Byte__F016345.MGF", "2024_2_38_07_PM_FileSize_1529844717_Byte_F016345.mzid", "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF-2.msf", "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF-2.mzML", and "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF.RAW". You can find data of pEhEx-HA (mock) in the second trial from "2024_5_13_15_PM_FileSize_1498194810_Byte_F016346.mzid_2024_5_13_15_PM_File_Size__1498194810__Byte__F016346.MGF", "2024_5_13_15_PM_FileSize_1498194810_Byte_F016346.mzid", "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA-2.msf", "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA-2.mzML", and "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA.RAW"

INSTRUMENT(S):

ORGANISM(S): Entamoeba Histolytica Hm-1:imss

TISSUE(S): Trophozoite

SUBMITTER: Misato Shimoyama  

LAB HEAD: Tomoyoshi Nozaki

PROVIDER: PXD068844 | Pride | 2026-05-16

REPOSITORIES: Pride