MET-G4_HepG2_Cell lysate_LC-MSMS

ABSTRACT: A biotin-labeled MET-G4-forming sequence was used as bait to capture proteins binding to MET-G4. In contrast, a biotin-labeled MET-G4-MUT sequence served as a control bait to exclude non-specific DNA-binding proteins. After incubation with cell lysate from HepG2 cells, the biotin-labeled sequences were immobilized on avidin agarose beads for protein enrichment. Subsequently, the enriched proteins were separated by SDS-PAGE gel electrophoresis and then subjected to LC-MS/MS analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Liver Cancer

SUBMITTER: Yipu Li  

LAB HEAD: Yipu Li

PROVIDER: PXD068846 | Pride | 2026-05-19

REPOSITORIES: Pride