Dataset Information

Integrated tissue and serum proteomics on magnet-guided photothermal therapy using Au/Fe/Au trilayer nanodiscs

ABSTRACT: Nanodiscs are emerging as promising nanomaterials for localized therapies as they enable magnetic targeting and near-infrared (NIR) laser–driven photothermal stimulation. In this study, we performed comprehensive proteomic analyses of both tissue and serum from mouse models to elucidate the effects of photothermal therapy using Au/Fe/Au trilayer nanodiscs (AuFeAuNDs) on both local and systemic protein regulation. Protein expression in tissue and serum was analyzed under six experimental exposure conditions: control, control plus laser irradiation, Au nanodiscs, AuFeAuNDs without magnetic induction, AuFeAuNDs with magnetic induction, and AuFeAuNDs with magnetic induction plus laser irradiation. Tissue proteomes showed enrichment of proteins involved in histone modification and transcriptional regulation, while serum proteomes revealed alterations in immune responses and coagulation pathways, with the combined AuFeAuNDs, magnet, and laser treatment promoting fibrinolysis. Fifty-two shared proteins across tissues and serum were implicated in ferroptosis, coagulation, and defense responses. Overall, these findings provide mechanistic insights into the biological impact and biosafety of nanodisc-mediated photothermal therapy, as well as highlight potential biomarkers for monitoring therapeutic responses.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Serum, Ct-26 Cell

SUBMITTER: Sun Young Lee

LAB HEAD: Jin Gyeong Son

PROVIDER: PXD068909 | Pride | 2025-10-15

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 92

Similar Datasets

Project description:We report that terahertz (THz) irradiation of mouse mesenchymal stem cells with a pulsed broadband (centered at 10 THz) source, or a single-frequency, 2.52 THz, (SF) laser source, both with weak average power (<1mW/cm2), results in specific heterogenic changes in gene expression. The insignificant differential expression of heat shock and stress related genes as well as our temperature measurements imply a non-thermal response. The microarray survey and RT-PCR experiments demonstrate that at different irradiation conditions distinct groups of genes are activated. Stem cells irradiated for 12 hours with the broadband THz source exhibit an accelerated differentiation toward adipose phenotype, while the 2-hour (broadband or SF) irradiation affects genes transcriptionally active in pluripotent stem cells. Phenotypic and gene expression differences suggest that the THz effect depends on irradiation parameters such as duration and type of THz source, and on the level of stem cell differentiation. Computer simulations of the core promoters of two pluripotency markers reveal association between gene upregulation and propensity for DNA breathing. We propose that THz radiation has potential for non-contact control of cellular gene expression. Growing in petri dish mouse mesenchymal stem cell cultures are irradiated for 2 hours with a single-frequency (2.52 THz) CW laser source. Three independent biological experiments were conducted. In each experiment, a control mMSC culture was placed adjacent to the irradiated sample, and the temperature was kept at 26-27M-BM-:C for both the control and the irradiated sample. Immediately after completing the irradiation, total RNA was extracted from the irradiated sample and the control, and microarrays were used to identify differential changes in gene expression between the irradiated sample and its respective control. In each experiment, the mMSC cultures were synchronized to be at the same differentiation time point immediately before the irradiation.

			Action	DRS
	Mouse_serum_with_AuFeAuNDs.mgf	Mgf
	Mouse_tissues_with_AuFeAuNDs.mgf	Mgf
	SDRF.xlsx	Xlsx
	Serum_1_Ctrl_1.tdf	Other
	Serum_1_Ctrl_1.tdf_bin	Other

Dataset Information

Integrated tissue and serum proteomics on magnet-guided photothermal therapy using Au/Fe/Au trilayer nanodiscs

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets