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HDX-MS analysis of p110a in complex with regulatory subunits

ABSTRACT: HDX-MS was used to map the changes in conformational dynamics in the catalytic subunit of PI3Ka, p110a, in complex with the following five reguatory subunits: p85a, p85b, p55g, p55a, p50a. Apo and pY activated states were also compared between the three splice variants encoded by PIK3R1. No significant changes were observed in p110a between any of the regulatory subunits in the apo state.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke

LAB HEAD: Dr. John E. Burke

PROVIDER: PXD069057 | Pride | 2025-11-20

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	07_05_24_IB7_A-MSMS.d.zip	Other
	07_15_25_IB8_A_MSMS.d.zip	Other
	IBHDX7_master_spreadsheet.xlsx	Xlsx
	IBHDX7_p110a-FASTA.txt	Txt
	IBHDX7_p110a_analysis_PRIDESubmission.hdx	Other

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1 - 5 of 13

Publications

Activity and dynamics of p110α are not differentially modulated by regulatory subunit isoforms.

Barlow-Busch Isobel I Walsh Emma E EE Nyvall Hunter G HG Burke John E JE

Advances in biological regulation 20251104

Class IA phosophoinositide kinases (PI3Ks) are master regulators of growth, metabolism, and immunity. The class IA PI3Ks are a heterodimer composed of a p110 catalytic subunit and one of five possible regulatory subunits (p85α, p85β, p55γ, p55α, p50α). The regulatory subunit plays critical roles in stability, inhibition, and activation of the p110 catalytic subunit. The p110α catalytic subunit frequently contains activating mutations in human cancer, with many of these mutations altering the int ...[more]

PMID: 41203468

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Activation loop phosphorylation tunes conformational dynamics underlying Pyk2 tyrosine kinase activation

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An HDX-MS optimised approach to characterise nanobodies as tools for biochemical and structural studies

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