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Identification of new RhoGDI2 interactants by co-IP and MS

ABSTRACT: A co-precipitation experimental model was developed to identify proteins potentially physically associated with RhoGDI2. Cells expressing RhoGDI2 tagged with FLAG® M2 were generated. This tag was placed at either the C- or N-terminus to reduce the probability of missing potential partners specific to the N- or C-terminal end domains of RhoGDI2. Tagged RhoGDI2 was immuno-precipitated with anti-FLAG® M2 antibody and co-precipitated proteins were identified by Mass Spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christophe Deroanne

LAB HEAD: Christophe F Deroanne

PROVIDER: PXD069111 | Pride | 2025-11-27

REPOSITORIES: Pride

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			Action	DRS
	DIA1_U2OS_CTR_replicate_1_01.d.zip	Other
	DIA1_U2OS_CTR_replicate_1_01_SEARCH.zip	Other
	DIA2_U2OS_C_Flag_replicate_1_02.d.zip	Other
	DIA2_U2OS_C_Flag_replicate_1_02_SEARCH.zip	Other
	DIA3_U2OS_N_Flag_replicate_1_03.d.zip	Other

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