Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Anabaena Cyanoglobule Proteomics

ABSTRACT: Cyanoglobules (CGs) are thylakoid-associated lipid droplets in cyanobacteria that share compositional and functional features with plastoglobules (PGs) of plant chloroplasts. However, their roles in stress physiology remain poorly defined, particularly in filamentous cyanobacteria. Here, we characterize the CG proteome in a non-nitrogen-fixing (ΔN) strain of Anabaena sp. PCC 7120 under nitrogen deprivation, providing an unbiased framework to examine CG responses without the confounding influence of heterocyst differentiation or diazotrophy. Proteomic analysis revealed a conserved CG proteome enriched in plastoglobule homologs, redox regulators (NDC1, GRXC2), and isoprenoid metabolism enzymes (NDX1), supporting roles in pigment turnover and stress adaptation.

INSTRUMENT(S):

ORGANISM(S): Anabaena Sp. Bta17

SUBMITTER: Peter Lundquist

LAB HEAD: Peter Lundquist

PROVIDER: PXD069172 | Pride | 2026-02-23

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	HNNBDT_1_A_N_Dep_ncbi.faa	Other
	MaxQuant_1.6.15.0.zip	Other
	MaxQuant_Output_Results.zip	Other
	hf2020061908.raw	Raw
	mqpar.xml	Xml

Items per page:

1 - 5 of 5

Publications

Cyanoglobule lipid droplet accumulation as a stress response to nitrogen starvation in a non-N2-fixing mutant strain of Anabaena sp. PCC 7120.

Susanto Febri A FA Hiser Carrie C Mahey Mohit M Lundquist Peter K PK

PloS one 20260220 2

Cyanoglobules are lipid droplets of cyanobacteria that share compositional and functional features with plastoglobules of plant chloroplasts. However, their roles in stress physiology remain poorly defined, particularly in filamentous cyanobacteria. Here, we characterize cyanoglobule dynamics and composition during nitrogen starvation in a non-diazotrophic derivative of Anabaena sp. PCC 7120. This experimental context enables analysis of sustained nitrogen deprivation in vegetative cells without ...[more]

PMID: 41719311

Similar Datasets

Transcriptional Regulation of Anabaena 7120 response to Nitrogen Deprivation

Project description:Here, we report the transcriptome of Anabaena sp. strain 7120, a cyanobacterium that forms specialized nitrogen-fixing cells called heterocysts. Our data suggests that cyanobacteria frequently have more complex transcripts than thought, with large 5' UTRs, numerous antisense transcripts, and multiple transcriptional start sites or processing sites. Four samples of total filament RNA were sequenced with Illumina 40bp reads using directional RNA sequencing (see the Illumina small RNA prep protocol). The samples are 0hr (vegetative cells grown in the presence of ammonia) and 6hr, 12hr, and 21hr cells (after nitrogen step down).

2011-06-22 | E-GEOD-26633 | biostudies-arrayexpress

Transcriptional Regulation of Anabaena 7120 response to Nitrogen Deprivation

2011-06-22 | GSE26633 | GEO

A new world of RNA-binding proteins in heterocyst-forming cyanobacteria.

Project description:RNA-binding proteins (RBPs) are central components of gene regulatory networks and RNA metabolism. The differentiation of nitrogen-fixing heterocysts in some filamentous cyanobacteria and their cooperation with vegetative cells in the same filament is one of the evolutionarily oldest cases of true multicellularity. Nostoc sp. PCC 7120 (Nostoc) is a model organism for filamentous cyanobacteria that differentiate heterocysts. Although hundreds of noncoding transcripts may be involved in this process, the RBPs that could cooperate with them are poorly characterized. We have used R-DeeP/GradR, a technique based on the differential sedimentation of RNA-protein complexes after RNase treatment in density gradients. All macromolecular complexes in the cell are fractionated by a sucrose gradient followed by high-resolution proteomics. We have compared gradients of RNase-treated with untreated samples in triplicates. If a protein was part of an RNA-protein complex, the complex should be disassembled after the RNase treatment and a shift of the protein position in the gradient should be observed (RNA-dependent proteins). Our approach yielded 333 RNA-dependent proteins. The results were compared to a bioinformatic prediction of RBPs and biochemical validation. We validated in vivo the RNA-binding capacity of 6 new RBPs. Some of these proteins were previously known to be involved in essential biological processes such as photosynthesis or heterocyst differentiation, but their association with RNA was previously unknown. In other cases, the function of the validated candidates was unknown. A knock-out strain of alr1700, a gene encoding a validated RNA-binding protein of unknown function, showed a deregulation of heterocyst differentiation, confirming the role of RBPs in regulating this cell differentiation process.

2024-11-08 | PXD050404 | Pride

Early events of the symbiotic interaction between Oryza sativa and Nostoc punctiforme revealed by a quantitative proteomic approach.

Project description:Plant-cyanobacteria symbiosis is considered one of the pivotal events in the history of life. In this symbiosis, the cyanobacterium provides to the plant fixed nitrogen compounds and plant hormones and, in return, the plant provides to the cyanobacterium fixed carbon. Despite the large knowledge in the physiology and ecology of plant-cyanobacteria symbioses, little is known about the molecular mechanisms involved in the crosstalk between partners. It has been shown recently that Nostoc punctiforme is able to stablish an endophytic symbiosis with Oryza sativa. This finding opens a door to explore this symbiotic interaction as a sustainable alternative to nitrogen fertilization of paddy fields. However, molecular mechanisms behind Oryza-Nostoc endosymbiosis are still not clarified. To gain further insights, an LC-MS/MS based label-free quantitative technique was used to evaluate the differential proteomics under N. punctiforme treatment vs. control plants at 1 day and 7 days. Differential expression profiling reveals a significant number of proteins to be down-regulated or missing in both partners, while others were more abundant or only expressed when both partners were in contact. In N. punctiforme, the differential protein expression was primarily connected to primary metabolism, signal transduction and perception, transport of substances and photosynthesis. In O. sativa, the differential protein expression was connected to a wide range of biological functions regulating carbon and nitrogen metabolism and response to biotic and abiotic stresses.

2023-03-10 | PXD022229 | Pride

Synechocystis sp. PCC 6803 proteome response to P-deficiency

Project description:Lipid droplets (LDs) are well integrated into multiple facets of cellular physiology and potentially represent an effective platform for the engineering of heterologous metabolic pathways. LDs of higher plant chloroplasts, known as plastoglobules (PGs), are critical mediators of stress tolerance through dynamic and reversible changes to morphology and molecular composition, however the dynamics and functional role(s) of cyanobacterial LDs are almost wholly unknown. Owing to their endosymbiotic origin as the progenitors of plant chloroplasts, we showed that cyanobacterial LDs designated as “cyanoglobule” share common characteristics with PGs, including molecular composition, structure, and their response under stress condition. We have monitored the dynamic accumulation of LDs, their protein, and lipid composition in response to phosphate deprivation. We are the first to describe cyanoglobule core proteome and lipidome in S6803 and their dynamics under P-deficiency. Our results showed that cyanoglobule share broadly similar protein and lipid compositions with plant plastoglobule, pointing to a central hub of cell lipid-protein metabolism and common role in stress adaptation, which then indicated their evolutionary relationship although specific differences may underscore an expansion of functions among higher plant PGs.

2025-05-21 | PXD051903 | Pride

Modulation of abiotic stress tolerance and photosynthesis of cyanobacterium Nostoc PCC 7120 through altered phosphoproteome mediated by Nucleoside diphosphate kinase

Project description:Nucleoside diphosphate kinase (Ndk/NDK/NDPK) is known to possess pleiotropic functions, one of which is that as a protein kinase, and known to be involved in stress tolerance in plants. The role of Ndk in the ancient cyanobacteria, also known as the progenitor of chloroplast, has not been investigated. To assess the role in Nostoc PCC 7120, a recombinant strain overexpressing Ndk, Anndk+ was generated. Phosphoproteomic analysis of Anndk+ and its comparison with that of the vector control AnpAM revealed differential phosphorylation at S/T/Y sites of proteins belonging to varied functional groups, with the majority being from those involved in photosynthesis. A total of 177 phosphopeptides and 117 phosphoproteins were identified, some of which have been identified as a phosphopeptide for the first time in any cyanobacteria. Compared to AnpAM, the Anndk+ cells exhibited (i) lower photosynthetic efficiency and electron transport rate at low PAR (photosynthetically active radiation), (ii) no change in photochemical quenching across PAR, (iii) but distinct non-photochemical quenching [zero Y(NPQ) and high Y(NO) in Anndk+ and high Y(NPQ) and low (NO) in AnpAM], and (iv) increased tolerance to -radiation, oxidative, salt and DCMU stresses. The possible role of the change in phosphoproteome of Nostoc upon overexpression of Ndk in modulating photosynthetic parameters and abiotic stress tolerance has been discussed. This is the first report on a wide physiological role for nucleoside diphosphate kinase in any cyanobacteria.

2025-05-06 | PXD050799 | Pride

The sRNA NsiR4 is involved in nitrogen assimilation control in cyanobacteria by targeting glutamine synthetase inactivating factor IF7

Project description:Glutamine synthetase (GS), a key enzyme in biological nitrogen assimilation, is regulated in multiple ways in response to varying nitrogen sources and levels. Here we show a small regulatory RNA, NsiR4 (nitrogen stress induced RNA 4), which plays an important role in the regulation of GS in cyanobacteria. NsiR4 expression in the unicellular Synechocystis sp. PCC 6803 and in the filamentous, nitrogen-fixing Anabaena sp. PCC 7120 is stimulated through nitrogen-limitation via NtcA, the global transcriptional regulator of genes involved in nitrogen metabolism. NsiR4 is widely conserved throughout the cyanobacterial phylum, suggesting a conserved function. In silico target prediction, transcriptome profiling upon pulse overexpression and site-directed mutagenesis experiments using a heterologous reporter system showed that NsiR4 interacts with the 5’UTR of gifA mRNA, which encodes glutamine synthetase inactivating factor IF7. In Synechocystis, we observed an inverse relationship between the levels of NsiR4 and the accumulation of IF7 in vivo. This NsiR4-dependent modulation of gifA (IF7) mRNA accumulation influenced the glutamine pool and thus NH4+ assimilation via glutamine synthetase. As a second target, we identified ssr1528, a hitherto uncharacterized nitrogen-regulated gene. Competition experiments between wild type and an NsiR4 knock-out mutant showed that the lack of NsiR4 led to decreased acclimation capabilities of Synechocystis towards oscillating nitrogen levels. These results suggest a role for NsiR4 in the regulation of nitrogen metabolism in cyanobacteria, especially for the adaptation to rapid changes in available nitrogen sources and concentrations. NsiR4 is the first identified bacterial sRNA regulating the primary assimilation of a macronutrient.

2015-10-20 | GSE73840 | GEO

Maize plastoglobule and thylakoid proteomes during water deficit and recovery

Project description:The maize plastoglobule proteome has not been reported in the literature, nor has its dynamic repsonse to water-deficit (i.e. drought) been investigated. To address this shortcoming, we have employed deep bottom-up proteomics of isolated plastoglobules, as well as isolated thylakoids, from 3-week-old maize seedlings experienceing a drought stress treatment. Maize plants were not watered for 17 days, during which a decline in photosynthetic parameters and leaf wilting and tip chlorosis was observed, followed by re-watering from which plants quickly recovered. Plastoglobules and thylakoods were isolated at six different time points during the stress treatment to monitor dynamic changes during entry itno and recovery from the stress. These siz time points are: 7 days without watering (dWW), 12 dWW, 16 dWW, 17 dWW, 1 day after re-watering (dRW), and 3 dRW. The 7 dWW time point represents the basal state prior to imposition of stress (plants are only watered once every 7 days), while the 17 dWW represents the peak stress time point. We find tailored changes to plastoglobule and thylakoid consistent with triggering of specific stress tolerance mechanisms, but no indication of senescence processes were observed. Our results provide the first public report of the maize plastoglobule proteome and its dynamic responses to water-deficit.

2025-10-17 | PXD050599 | Pride

RNA-seq of Nostoc sp. PCC 7120 (Anabaena sp. PCC 7120) wild-type strain, hetZ mutant and hetP mutant

Project description:Total RNA was extracted from Anabaena 7120, hetZ mutant and hetP mutant at 24 h after nitrogen stepdown with two independent biological replicates. To maintain the integrity of coding RNAs, total RNA were sequenced without ribosomal RNA elimination. Strand-specific RNA-Seq libraries were prepared and sequenced using the Illumina HiSeq 2500 sequencing instrument to generate paired-end reads with length of 125 bp.

2018-02-19 | E-MTAB-6459 | biostudies-arrayexpress

Differentially forms of nitrogen regulates the response of Microcystis aeruginosa to nanoplastics under environmentally relevant nitrogen concentration

Project description:As an essential primary producer, cyanobacteria play an important role in the global cycle for both carbon and nitrogen in the ecosystems. Though the influence of nanoplastics on the carbon metabolism of cyanobacteria, especial Microcystis aeruginosa, a dominant species causing cyanobacterial blooms, is well studied, little is known about nanoplastics affecting the nitrogen metabolism.

2023-09-01 | GSE214536 | GEO