Assembly and cell-free expression of a partial genome for the synthetic cell

ABSTRACT: We designed and assembled a multicistronic DNA plasmid containing multiple genes that are relevant for a synthetic cell. This partial minimal synthetic genome was expressed in the recombinant PURE system. Cell-free biosynthesis of all encoded proteins was validated by liquid chromatography-mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli Bacillus Phage Phi29 Anaplasma Marginale Aequorea Victoria

SUBMITTER: Alexandre STELLA  

LAB HEAD: Odile Schiltz

PROVIDER: PXD069407 | Pride | 2026-10-28

REPOSITORIES: Pride