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Phosphoprofile reorganization of the actin binding protein Drebrin during long term depression

ABSTRACT: Drebrin (DBN), an actin-binding protein critical for the structural integrity and function of dendritic spines, is highly phosphorylated at steady state in neurons. Here, we investigate the phosphorylation dynamics of DBN in the context of chemically induced long-term depression (cLTD), a synaptic plasticity model mimicking activity-dependent weakening of synapses. Using biochemical analyses and mass spectrometry analyses, we show that DBN undergoes rapid and robust changes in phosphorylation following cLTD induction. Notably, cLTD triggers a marked decrease in many DBN phosphorylation sites, accompanied by proteolytic cleavage of the protein, suggesting a tightly regulated mechanism linking post-translational modification to structural remodelling of the synapse. Our findings highlight the dynamic regulation of DBN by phosphorylation during synaptic depression and support its potential role as a modulator of activity-dependent synaptic plasticity.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Synapse

DISEASE(S): Mental Depression

SUBMITTER: Marieluise Kirchner

LAB HEAD: Philipp Mertins

PROVIDER: PXD069538 | Pride | 2025-10-17

REPOSITORIES: Pride

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			Action	DRS
	PhosphoSTYSites.txt	Txt
	Solo_20230825_SN_MMtmtproph_Eickh_Ph_01.raw	Raw
	Solo_20230825_SN_MMtmtproph_Eickh_Ph_02.raw	Raw
	Solo_20230825_SN_MMtmtproph_Eickh_Ph_03.raw	Raw
	Solo_20230825_SN_MMtmtproph_Eickh_Ph_04.raw	Raw

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