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The complementary activity of two carbohydrate esterases dictates deacetylation of β-mannans by the human gut microbe Bacteroides cellulosilyticus

ABSTRACT: The project aimed at determining the enzymatic machinery deployed by Bacteroides cellulosilyticus to remove acetylations from complex β-mannans

INSTRUMENT(S):

ORGANISM(S): Bacteroides Cellulosilyticus Dsm 14838

TISSUE(S): Cell Culture

SUBMITTER: Sabina Leanti La Rosa

LAB HEAD: Sabina Leanti

PROVIDER: PXD069567 | Pride | 2026-05-18

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	AcGGM1.tar.gz	Other
	AcGGM2.tar.gz	Other
	AcGGM3.tar.gz	Other
	FragPipeAnalysis.tar	Other
	Glucose1.tar.gz	Other

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The Human Gut Firmicute Roseburia intestinalis is a Primary Degrader of Dietary β-Mannans

Project description:β-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in β-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available β-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, β-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using β-mannan-based therapeutic interventions.

2019-02-21 | PXD012448 | Pride