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Regulation by hypusination of the proteome of GECs during H. pylori infection

ABSTRACT: Mice with or without specific deletion of the gene Dhps in intestinal epithelial cells were infected (n = 2 per genotype) or not (n = 2 per genotype) with Helicobacter pylori PMSS1 for 8 weeks. Proteins from isolated gastric epithelial cells (GECs) were extracted and analyzed using a TMT-based proteomics strategy.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Gastric Epithelial Cell, Epithelial Cell Of Stomach

DISEASE(S): Gastritis

SUBMITTER: Kristie Lindsey Rose

LAB HEAD: Kristie Lindsey Rose

PROVIDER: PXD069622 | Pride | 2026-06-01

REPOSITORIES: Pride

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			Action	DRS
	checksum.txt	Txt
	pEX2_pp_6596_20250728_PeptideGroups_PD.xlsx	Xlsx
	pEX2_pp_6596_20250728_Proteins_PD.xlsx	Xlsx
	pEX2_pp_6596_20250728_TMT4Plex_BRP_F1_0pt5uL_1.raw	Raw
	pEX2_pp_6596_20250728_TMT4Plex_BRP_F2_1pt2uL_2.raw	Raw

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Publications

Epithelial hypusination regulates Helicobacter pylori-induced gastric inflammation.

Gobert Alain P AP McNamara Kara M KM Hawkins Caroline V CV Asim Mohammad M Barry Daniel P DP Delgado Alberto G AG Rose Kristie L KL Patel Purvi P Tyree Regina N RN Carson Kate S KS Coburn Lori A LA Piazuelo M Blanca MB Wilson Keith T KT

Amino acids 20260202 1

Hypusine is a unique amino acid synthesized on the eukaryotic initiation factor 5 A (EIF5A) from the polyamine spermidine by deoxyhypusine synthase (DHPS). Hypusination of EIF5A plays a key role in translation. Here, we examined the contribution of the epithelial hypusination pathway to gastric inflammation induced by Helicobacter pylori. Immunohistochemical analyses revealed increased expression of DHPS and hypusinated EIF5A (EIF5A<sup>Hyp</sup>) in the gastric mucosa of patients with H. pylori ...[more]

PMID: 41627564

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