Proteomic Analysis of Downstream Proteins Regulated by THRAP3 in THP-1 Cells

ABSTRACT: This study aimed to analyze the downstream proteins regulated by THRAP3 in THP-1 cells using proteomics. THP-1 cells were divided into control and KO groups (with 3 biological replicates each, transfected with sgNC or sgTHRAP3 respectively). After cell lysis, trypsin digestion, Tandem Mass Tags labeling, fractionation, and resuspension, peptide fractions were separated via LC and analyzed by MS on a Q-Exactive HF Orbitrap mass spectrometer. MS data were interpreted with Percolator v.355 to identify UniProt sequences with a 1% FDR.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte, Cell Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Pan Chen  

LAB HEAD: Pan Chen

PROVIDER: PXD069724 | Pride | 2025-12-11

REPOSITORIES: Pride