Dataset Information

Proteomic Analysis of Downstream Proteins Regulated by THRAP3 in THP-1 Cells

ABSTRACT: This study aimed to analyze the downstream proteins regulated by THRAP3 in THP-1 cells using proteomics. THP-1 cells were divided into control and KO groups (with 3 biological replicates each, transfected with sgNC or sgTHRAP3 respectively). After cell lysis, trypsin digestion, Tandem Mass Tags labeling, fractionation, and resuspension, peptide fractions were separated via LC and analyzed by MS on a Q-Exactive HF Orbitrap mass spectrometer. MS data were interpreted with Percolator v.355 to identify UniProt sequences with a 1% FDR.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte, Cell Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Pan Chen

LAB HEAD: Pan Chen

PROVIDER: PXD069724 | Pride | 2025-12-11

REPOSITORIES: Pride

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Publications

THRAP3 promotes ferroptosis resistance in acute myelocytic leukemia through SLU7-mediated alternative splicing of GIT2.

Wang Dan D Wu Zhixiang Z Liu Siyang S He Jing J Zhang Haixia H Chen Pan P Yang Minghua M

Nature communications 20251201 1

Induction of ferroptosis is a potential strategy to eliminate chemotherapy-resistant acute myeloid leukemia (AML) cells. Here, we investigate the role and mechanism of thyroid hormone receptor-associated protein 3 (THRAP3) in ferroptosis of AML cells. We show that high expression of THRAP3 is correlated with a poor prognosis in AML patients. THRAP3 knockdown suppresses AML cell proliferation, and delays orthotopic and subcutaneous tumor growth in male mice; however, THRAP3 overexpression exerts ...[more]

PMID: 41326370

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			Action	DRS
	BP20230605_PSMs.txt	Txt
	BP20230605_PeptideGroups.txt	Txt
	BP20230605_Proteins.txt	Txt
	KO-1.raw	Raw
	KO-2.raw	Raw