Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RHOT proteins link mitochondrial motility to cardiomyocyte sarcomere maturation

ABSTRACT: Proteome analysis of RHOT1/2 interaction partners based on affinity purification.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Felix Polten

LAB HEAD: Christian Riehle

PROVIDER: PXD069743 | Pride | 2026-04-30

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Interaction_GFP_R1_F1.raw	Raw
	Interaction_GFP_R1_F2.raw	Raw
	Interaction_GFP_R2_F1.raw	Raw
	Interaction_GFP_R2_F2.raw	Raw
	Interaction_GFP_R3_F1.raw	Raw

Items per page:

1 - 5 of 20

Publications

RHOT Proteins Link Mitochondrial Motility to Cardiomyocyte Sarcomere Maturation.

Froese Natali N Shymotiuk Ivanna I Froese Alexander A Polten Felix F Jablinski Jonas J Scholz Tim T Korf-Klingebiel Mortimer M Werlein Christopher C Galuppo Paolo P Gigina Anna A Wihler Katharina K Schneider Johanna J Erschow Sergej S Heimerl Maren M Szaroszyk Malgorzata M Hegermann Jan J Wrede Christoph C Schweitzer Theresa T Pich Andreas A Geffers Robert R Ricke-Hoch Melanie M Kühnel Mark P MP Jonigk Danny D DD Wende Adam R AR Abel E Dale ED Nikolaev Viacheslav O VO Wollert Kai C KC Bauersachs Johann J Riehle Christian C

Circulation research 20260316 9

<h4>Background</h4>Cardiomyocyte mitochondria align with sarcomeres during heart development. Mitochondrial motility is controlled by RHOT (ras homolog family member T) 1 and RHOT2. RHOT1 and RHOT2 are atypical Rho-like small GTPases that are anchored to the outer mitochondrial membrane and couple mitochondria to kinesin and dynein motors. We hypothesized that RHOT protein expression and mitochondrial motility are required for mitochondrial positioning during cardiomyocyte development.<h4>Method ...[more]

PMID: 41834725

Similar Datasets

MIR-204 target genes

Project description:The aim of this study is to discover genes regulated by miR-204. Differential gene expression in HEK-293 cells transfected with miR-204-mimic compared to HEK-293 cells transfected with control oligo (HEK-293 control) was analyzed using the Agilent Human Whole Genome 4x44K gene expression array (Agilent Technologies, Santa Clara, CA). HEK-293 cells were transfected with either miR-204 or a control, and gene expression was analyzed using the Agilent Human Whole Genome 4x44K array. A dye-swap was performed.

2012-03-30 | E-GEOD-28400 | biostudies-arrayexpress

Transcription profiling by array of cells starved of leucine in the presence or absence of a dominant negative HSF1 mutant (dnHSF1, HSF379).

Project description:Cells were starved for leucine in the presence or absence of a dominant negative HSF1 mutant (dnHSF1, HSF379). Changes in transcriptome relative to cells cultured with leucine were determined. The experiment shows the effect of dnHSF on the amino acid starvation response.

2012-12-11 | E-MEXP-3563 | biostudies-arrayexpress

Gene expression of HEK 293 cells transfected with Vector (PCMV), Aire, or MBD-VP16

Project description:HEK 293 cells were transiently transfected with plasmids expressing Vector only(PCMV), Aire, or MBD-VP16 with the goal of comparing the global gene expression profiles in the Aire and MBD-VP16 groups We used microarrays to detail the global gene expression profile. HEK 293 cells were transiently transfected with plasmids expressing Vector only(PCMV), Aire, or MBD-VP16 and 72 hrs post transfection total RNA was collected for microarray analysis.

2013-11-21 | E-GEOD-52546 | biostudies-arrayexpress

miRNA and Wilms' tumor

Project description:This SuperSeries is composed of the following subset Series: GSE17342: The role of miRNA in Wilms' tumorigenesis GSE28397: Copy number alteration in Wilms' tumor with custom-designed miRNA probes GSE28400: MIR-204 target gene Refer to individual Series

2012-03-30 | E-GEOD-28401 | biostudies-arrayexpress

Asparagine hydroxylation of the UBA domain in Cezanne (OTUD7B)

Project description:by DUBs need to be tightly controlled. Here, we identify asparagine hydroxylation as a novel posttranslational modification involved in the regulation of Cezanne (OTUD7B), a DUB that controls key cellular functions and signaling pathways. We demonstrate that Cezanne is a substrate for FIH1- and oxygen-dependent asparagine hydroxylation. FIH1 modifies Asn35 within the uncharacterized N-terminal ubiquitin-associated (UBA)-like domain of Cezanne (UBACez), which lacks conserved UBA domain properties. We show for the first time that UBACez binds Lys11-, Lys48-, Lys63- and Met1-linked ubiquitin chains in vitro, and thereby establish UBACez as a functional ubiquitin-binding domain. We reveal that interaction of UBACez with ubiquitin is mediated via a non-canonical surface, and that hydroxylation of Asn35 inhibits ubiquitin binding. Recently, it has been suggested that recruitment of Cezanne to specific target proteins depends on UBACez. Our data show that UBACez can indeed fulfil this role as regulatory domain by binding differently linked ubiquitin chains and that this interaction with ubiquitin, and thus modified substrates, can be modulated by asparagine hydroxylation.

2020-01-24 | PXD015216 | Pride

PQBP1-associated proteins spectrums in HEK-293 cells

Project description:Our study shows that deletion of the alternative splicing regulator PQBP1 in striatal progenitors resulted in defective striatal development due to impaired neurogenesis of spiny projection neurons (SPNs). Therefore, we further reveal that PQBP1 associated with components in splicing machinery by LC-MS/MS. These findings identify PQBP1 as a novel regulator in balancing striatal progenitor proliferation and differentiation.

2023-03-16 | PXD040439 | Pride

Definition of the fibroblast adhesome using multiplexed proximity biotinylation

Project description:Integrin adhesion complexes (IACs) bridge the extracellular matrix to the actin cytoskeleton and transduce signals in response to both chemical and mechanical cues. The composition, interactions, stoichiometry and topological organisation of proteins within IACs are not fully understood. To address this gap, we used multiplexed proximity biotinylation (BioID) to generate an in situ, proximity-dependent adhesome. Integration of the interactomes of 16 IAC-associated baits revealed a network of 147 proteins with 361 proximity interactions. Candidates with underappreciated roles in adhesion were identified, in addition to established IAC components. Bioinformatic analysis revealed five clusters of IAC baits that link to common groups of prey, and which therefore may represent functional modules. The five clusters, and their spatial associations, are consistent with current models of IAC interaction networks and stratification. This study provides a resource to examine proximal relationships within IACs on a global level.

2020-06-01 | PXD017241 | Pride

NOD2 and desease associated variant NOD2-L1007fsinsC dependent genomewide transcriptional regulation in stable Flp-In HEK cells

Project description:NOD2 is an intracellular receptor for the bacterial cell wall component muramyl dipeptide (MDP) and variants of NOD2 are associated with chronic inflammatory diseases of barrier organs e.g. Crohn disease, asthma and atopic eczema. It is known that activation of NOD2 induces a variety of inflammatory and antibacterial factors. The exact transcriptomal signatures that define the cellular programs downstream of NOD2 activation and the influence of the Crohn-associated variant L1007fsinsC are yet to be defined. To describe the MDP-induced activation program, we analyzed the transcriptomal reactions of isogenic HEK293 cells expressing NOD2wt or NOD2L1007fsinsC to stimulation with MDP. Importantly, a clear loss-of-function could be observed in the cells carrying the Crohn-associated variant L1007fsinsC, while the NOD2wt cells showed differential regulation of growth factors, chemokines and several antagonists of NF-κB, e.g. TNFAIP3 (A20) and IER3. To elucidate the MDP-induced activation program we generated isogenic HEK293 cells stably expressing wildtype NOD2 or NOD2L1007fsinsC using a FRT-recombinase based approach. Cells carrying the inserted vector cassette were used as controls (mock-transfectant). To comprehensively analyze NOD2-mediated innate immune responses we analyzed transcriptomal signature patterns using genome-wide cDNA microarrays. Samples were harvested from cell cultures under normal growth conditions 0 h, 2 h and 6 h after MDP‑ stimulation of the cells.

2013-01-01 | E-GEOD-22611 | biostudies-arrayexpress

Aire co-opts the repressive ATF7ip/MBD1 protein complex for the induction of immune tolerance

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-11-21 | E-GEOD-52548 | biostudies-arrayexpress

effect of expression of a dominant negative HSF1 mutant

Project description:Expression of a dominant negative HSF1 mutant (HSF379) was induced in HEK293 cells and the effect of the transcriptome of non-stressed cells was monitored

2012-03-01 | E-MEXP-3561 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data