Dataset Information

Secretome analysis of primary CLL cells

ABSTRACT: Secretome analysis of primary CLL cells co-cultured in serum-free IMDM medium with either EL08 stroma cells or YK6 follicular dendritic cells overexpressing CD40L and interleukin 21 (“FDC CD40L IL21”).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell Line

DISEASE(S): Acute Leukemia

SUBMITTER: Piero Giansanti

LAB HEAD: Piero Giansanti

PROVIDER: PXD069904 | Pride | 2026-01-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Exp1_0306_R0104-01_S004903_L_DIA_01.raw	Raw
	Exp1_0306_R0104-01_S004904_L_DIA_01.raw	Raw
	Exp1_0306_R0104-01_S004905_L_DIA_01.raw	Raw
	Exp1_0306_R0104-01_S004906_L_DIA_01.raw	Raw
	Exp1_0306_R0104-01_S004907_L_DIA_01.raw	Raw

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Publications

Strong constitutive NF-κB signaling in B cells drives SLL/CLL-like lymphomagenesis and overcomes microenvironmental dependencies.

Soberón Valeria V Osswald Lena L Moore Andrew A Sosnowska Dominika D Swinerd Gene G Chen Jingyu J Baygün Seren S Diehl Carina C Seyhan Gönül G Kraus Laura L Gölling Vanessa V Trapp Ricarda R O'Neill Thomas J TJ Bortoluzzi Sabrina S Kovacs Daniel D Ammon Tim T Singroul Pankaj P Hubarzhevska Yuliia Y Öllinger Rupert R Mueller Sebastian S Baranov Olga O Giansanti Piero P Gillhuber Felix F Grath Sonja S Weigert Oliver O Rosenwald Andreas A Sasaki Yoshiteru Y Rajewsky Klaus K Steiger Katja K Bassermann Florian F Rad Roland R Krappmann Daniel D Ringshausen Ingo I Schmidt-Supprian Marc M

Leukemia 20260116

Aberrant activation of NF-κB transcription factors is a hallmark of human lymphomas. Most lymphoma-intrinsic as well as microenvironment-induced NF-κB activation occurs upstream of the key kinase IKK2, therefore affecting additional pathways. Here, we show that canonical NF-κB signaling in mouse B cells, induced through the expression of one or two copies of a constitutively active IKK2 variant, dose-dependently drives lymphomagenesis. The observed phenotype and stereotypic B cell receptor clona ...[more]

PMID: 41545700

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Project description:Background: Gluten proteins from wheat, rye, and barley play a substantial role in human nutrition. At the same time, they can trigger several different immune reactions. This, together with their influence on the quality of grain products and their emerging role as biomaterials, makes them an interesting target for further study. The proteins' propensity for aggregation challenges heterologous eukaryotic production systems. The yeast Komagataella phaffii has demonstrated excellent qualities as a production host for heterologous proteins and was therefore investigated as a platform strain.Results: A gene coding for the rye (Secale cereale) prolamin 75k γ-secalin was cloned and inserted into K. phaffii; protein expression was verified via mass spectrometry and immunoblotting and quantified via ELISA. Different parameters were investigated regarding their effect on target protein production and endoplasmic reticulum (ER) homeostasis, including the induction temperature and co- and post-translational import into the ER. At 28°C, the cells produced 1.69-fold more 75k γ-secalin than at 20°C. The introduction of the MATα-pro-region, in conjunction with either the MATα-pre- or OST1-pre-signal, led to significantly lower 75k γ-secalin accumulation, 0.20- and 0.18-fold, respectively. No mutant showed significant changes in the unfolded protein response compared to a non-producing strain.Conclusions: K. phaffii is a suitable host for prolamin production. The absence of a significant unfolded protein response during 75k γ-secalin expression indicates little challenge of ER-homeostasis by the aggregation-prone protein. It underscores K. phaffii's imminent role in protein production. The significantly decreased protein yield through the common protein secretion leader component MATα-pro demonstrates the need for further investigation into the role of secretion signals in optimizing K. phaffii as a production platform for repetitive, aggregation-prone proteins.