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Phosphatidylethanolamine Reprograms Macrophage Immunometabolism and Attenuates LPS-Driven Inflammation

ABSTRACT: This study investigates how distinct phosphatidylethanolamine (PE) species(PE 18:0/20:4 and PE 18:0/22:6) modulate the proteome and metabolome profile of RAW 264.7 macrophages under resting and LPS-stimulated conditions (M1-like macrophages).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Tatiana Maurício

LAB HEAD: Pedro Domingues

PROVIDER: PXD069938 | Pride | 2026-05-06

REPOSITORIES: Pride

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			Action	DRS
	RAW_C1.raw	Raw
	RAW_C2.raw	Raw
	RAW_C3.raw	Raw
	RAW_C4.raw	Raw
	RAW_C5.raw	Raw

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CstF-64 Influenced Gene Expression

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EFO: In preparation - Early Metabolic Predictors of Type 2 Diabetes Mellitus in Normoglycemic Adults: Insights from Six-Year Prospective Cohort Study

Project description:<p>INTRODUCTION: Type 2 Diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance and hyperglycemia, often preceded by latent metabolic disruptions. Early detection of metabolic alterations can facilitate timely intervention to delay or prevent T2DM onset. Metabolic profiling offers a useful tool to identify novel disease biomarkers and metabolic alterations before clinical manifestations. OBJECTIVE: This study aimed to identify early metabolic alterations and potential biomarkers predictive of T2DM in a cohort of healthy normoglycemic participants over a six-year follow-up. METHODS: A cohort of 94 healthy participants, both men and women aged 18-40, was studied at six-year intervals. Clinical and biochemical parameters were measured, and LC/MS/MS-based untargeted metabolomics analysis of plasma was performed at baseline and follow-up. RESULTS: At follow-up, 9 participants developed T2DM, 51 had prediabetes and 34 remained normoglycemic. Increasing insulin resistance and elevated future T2DM risk were observed in both the Prediabetes and Normal groups. Metabolomics analysis identified Phosphatidylethanolamine (PE) (20:3_18:0), 3beta,7alpha-Dihydroxy-5-cholestenoate and Tridecanoic acid – as having good predictive capacity for future T2DM risk at baseline with alterations in Phosphatidylethanolamine (PE) (20:3_18:0), and Tridecanoic acid persisting at follow-up. CONCLUSION: The study highlights the potential of metabolomics in identifying early metabolic predictors of T2DM, emphasizing the need for early interventions in healthy normoglycemic young adults.</p>

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