Dataset Information

P. aeruginosa proteomics under the compound 7

ABSTRACT: We used the compond 7 developped by Jianwei Chen's group in P. aeruginosa under different concentrations (blank, 8*MIC and 16*MIC). Then we conducted the differential proteomics testing.

INSTRUMENT(S):

ORGANISM(S): Pseudomonas Aeruginosa Pao1

SUBMITTER: Jiangwei Pan

LAB HEAD: Jiangwei Chen

PROVIDER: PXD069942 | Pride | 2026-01-17

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	16MIC-Astral-1_8_FPEP240106158-1A.raw	Raw
	8MIC-Astral-1_8_FPEP240106155-1A.raw	Raw
	BLANK-Astral-1_8_FPEP240106152-1A.raw	Raw
	checksum.txt	Txt
	report.pg_matrix.tsv	Tabular

Items per page:

1 - 5 of 6

Similar Datasets

Project description:Age-related bone degeneration is driven by structural deterioration, chronic inflammation, and impaired regenerative capacity, leading to an increased incidence of osteoarticular disorders. Mesenchymal stromal/stem cells (MSCs) have emerged as promising therapeutic candidates due to their immunomodulatory and regenerative properties, mediated largely through their secretome and extracellular vesicles (EVs). However, the therapeutic efficacy of MSC-derived products depends on both the cell source and the conditioning stimuli to which MSCs are exposed. In this study, we performed a comparative proteomic and exosomal microRNA (miRNA) profiling of secretomes derived from placenta-derived MSCs (PSCs) and adipose-derived MSCs (ASCs), and assessed the impact of priming strategies, including hypoxia, interferon-gamma (IFNγ), and interleukin-1 beta (IL1β), on paracrine properties of these cells. Proteomic analysis identified over 7,000 proteins, with PSC secretome enriched in pathways related to osteogenesis, chondrogenesis, extracellular matrix organization, angiogenesis, and immune regulation, whereas ASC secretome displayed limited enrichment in these processes. Functional scoring highlighted IL1β priming as the most effective strategy to enhance osteochondral and immunomodulatory protein signatures in PSCs. Differently, IFNγ priming selectively expanded the repertoire of exosomal miRNAs, with enrichment in signaling networks including Wnt/β-catenin, TGF-β, NF-κB, and T cell receptor pathways, underscoring its role in fine-tuning immune and regenerative functions. Together, these findings reveal that PSCs secrete a broader and more functionally relevant spectrum of bioactive molecules for osteoarticular applications compared with ASCs. Moreover, distinct priming strategies can differentially modulate their paracrine outputs. IL1β primarily enhances protein-driven regenerative and immunomodulatory activity, whereas IFNγ promotes a functionally enriched exosomal miRNA cargo. These results support PSC-derived secretomes as versatile candidates for next-generation, cell-free therapies in osteoarticular disease.

Dataset Information

P. aeruginosa proteomics under the compound 7

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets