Size-exclusion chromatography of soluble chloroplast proteins from Nicotiana tabacum (tobacco)

ABSTRACT: Maturase K (MatK) is the only splicing factor encoded in the chloroplast genomes of land plants. Here we used a transplastomic Nicotiana tabacum line, in which the plastidial MatK gene is C-terminally tagged with a 3x-HA tag, to conduct size-exclusion chromatography of soluble protein extracts enriched for chloroplast proteins. Fractions 5 and 6, in which MatK-HA was found most abundant by western blot analysis, were subjected to mass spectrometry analysis.

INSTRUMENT(S):

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Leaf

SUBMITTER: Barbara Pfister  

LAB HEAD: Samuel C. Zeeman

PROVIDER: PXD069946 | Pride | 2026-02-10

REPOSITORIES: Pride