Proteomics

Dataset Information

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Proteomic Analysis of the Differential Response of Pseudomonas aeruginosa and Staphylococcus aureus to Lacticaseibacillus rhamnosus Cell-Free Supernatant and Lactic Acid


ABSTRACT: This project investigates the proteomic response of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus to postbiotic metabolites derived from Lacticaseibacillus rhamnosus. Bacterial cultures were exposed to sub-MIC concentrations of L. rhamnosus cell-free supernatant (CFS) or lactic acid (LA) for 6 h, and intracellular proteins were extracted for quantitative label-free proteomics using HPLC–MS/MS. The dataset includes RAW mass spectrometry data and MaxQuant output files. The aim of this study is to compare stress responses triggered by complex postbiotic mixtures versus LA alone, focusing on antimicrobial and antibiofilm potential.

INSTRUMENT(S):

ORGANISM(S): Bacteria Staphylococcus Aureus Pseudomonas Aeruginosa Pao1

SUBMITTER: Marta Bianchi  

LAB HEAD: Kevin Kavanagh

PROVIDER: PXD069979 | Pride | 2026-01-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
P.aeruginosa_CFS_B.raw Raw
P.aeruginosa_CFS_C.raw Raw
P.aeruginosa_CFS_D.raw Raw
P.aeruginosa_LA_B.raw Raw
P.aeruginosa_LA_C.raw Raw
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Publications

Proteomic Analysis of the Differential Response of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> to <i>Lacticaseibacillus rhamnosus</i> Cell-Free Supernatant and Lactic Acid.

Bianchi Marta M   Maisetta Giuseppantonio G   Esin Semih S   Batoni Giovanna G   Kavanagh Kevin K  

Antibiotics (Basel, Switzerland) 20251215 12


<b>Background/Objectives:</b> Postbiotics derived from lactic acid bacteria are emerging as promising antimicrobial agents due to their antibacterial, antibiofilm, and immunomodulatory properties. Among their metabolites, lactic acid (LA) is thought to play a major role in antimicrobial activity. This study investigated the proteomic response of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> to <i>Lacticaseibacillus rhamnosus</i> cell-free supernatant (CFS) and compared it with t  ...[more]

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