A protocol for robust discovery proteomics using nano liquid chromatography using pillar-array column technology and high-resolution mass spectrometry with data- independent acquisition

ABSTRACT: The project establishes an easy-to-follow protocol to set up bottom-up proteomics experiments using state-of-the-art pillar-array columns and mass spectrometry. The protocol touches upon key points of the method development, discusses the trade-off between proteome depth and analysis time and demonstrates short-term robustness.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Dániel Papp  

LAB HEAD: Sebastiaan Eeltink

PROVIDER: PXD069993 | Pride | 2026-01-13

REPOSITORIES: Pride