Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Guard Cell-Enriched Phosphoproteome from open and closed stomata

ABSTRACT: Control of the stomatal aperture is multifaceted, involving a complex interplay of environmental cues and intracellular signaling pathways. It is well established that changes in ion gradients drive water movement into and out of the guard cell, thereby altering cell volume and modulating the opening or closing of the stomatal pore. These rapid responses are often regulated by phosphorylation cascades to efficiently transmit environmental status and either reduce water loss or enhance carbon assimilation. Here, we investigated the regulation of stomatal opening and closing by generating a proteome and phosphoproteome of guard cell-enriched tissue. The guard cell-enriched tissue with closed stomata showed greater levels of phosphorylation of proteins related to endomembrane trafficking and vacuoles when compared to both whole leaf tissue with closed stomata and guard cell-enriched tissue with open stomata. These results support the hypothesis that phosphorylation of endomembrane proteins may contribute to the regulation of stomatal movements.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Scott Lyons

LAB HEAD: Marcela Rojas-Pierce

PROVIDER: PXD070005 | Pride | 2026-05-22

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20240405_OE480_PC1244-Rojas-Pierce_TMT10_prot-F1.raw	Raw
	20240405_OE480_PC1244-Rojas-Pierce_TMT10_prot-F10.raw	Raw
	20240405_OE480_PC1244-Rojas-Pierce_TMT10_prot-F11.raw	Raw
	20240405_OE480_PC1244-Rojas-Pierce_TMT10_prot-F12.raw	Raw
	20240405_OE480_PC1244-Rojas-Pierce_TMT10_prot-F13.raw	Raw

Items per page:

1 - 5 of 37

Similar Datasets

Gene Expression in Arabidopsis thaliana Guard Cells

Project description:Stomata are formed by a pair of specialized guard cells that control the opening and closing of the stomatal pores on the leaf surface. These pores are the main route for microbe penetration into leaves. However, plants show a remarkable ability to close the pore when sensing the presence of microbial invaders; a phenomenon recently described as stomatal immunity. This study was initiated to understand the transcriptional regulation of this early plant defense against pathogens.

2013-07-26 | E-MTAB-4420 | biostudies-arrayexpress

Microarray analysis of scap1 mutant and wild type in Arabidopsis thaliana guard cell protoplast

Project description:Stomata are highly specialized organs which consist of pairs of guard cells and regulate gas and water vapor exchange in plants. While early stages of guard cell differentiation have been described and were interpreted in analogy to processes of cell type differentiation in animals, the downstream development of functional stomatal guard cells remains poorly understood. We have isolated an Arabidopsis mutant, scap1 (stomatal carpenter 1), that develops irregularly shaped guard cells and lacks the ability to control stomatal aperture, including CO2-induced stomatal closing and light-induced stomatal opening. SCAP1 was identified as a plant-specific Dof-type transcription factor expressed in maturing guard cells but not in guard mother cells. SCAP1 regulates the expression of genes encoding key elements of stomatal functioning and morphogenesis, such as a K+ channel protein, MYB60 transcription factor, and pectin methyl esterase. Consequently, ion homeostasis was disturbed in scap1 guard cells, and esterification of extracellular pectins was impaired so that the cell walls lining the pores did not mature normally. We conclude that SCAP1 regulates essential processes of stomatal guard cell maturation and functions as a key transcription factor regulating the final stages of guard cell differentiation.

2013-02-28 | GSE43964 | GEO

Microarray analysis of scap1 mutant and wild type in Arabidopsis thaliana guard cell protoplast

2013-02-28 | E-GEOD-43964 | biostudies-arrayexpress

MPK4 interacting proteins in guard cells through AP-MS

Project description:Plants are often challenged by numerous biotic and abiotic environmental stresses. They have evolved efficient signaling networks in response to the environmental challenges, such as pathogen invasion, drought, CO2 changes. Guard cells are specialized epidermal cells enclosing pores on leaf surface, aka, stomata. Stomatal guard cells are at the frontline of biotic and abiotic stress responses. The opening and closing of stomata are regulated by a sophisticated intracellular signaling networks. Mitogen-activated protein kinase 4 (MPK4) was first identified as negative regulator in systemic acquired resistance (SAR). It was also play important roles in cytokinesis, reproduction, and photosynthesis. MPK4 has higher expression levels in plant guard cells than in mesophyll cells. Arabidopsis mpk4 mutant has higher levels of salicylic acid (SA) and reactive oxygen species (ROS), which are related with stomatal movement. The specific function of MPK4 in guard cells is unknown. In order to understand MPK4 functions in guard cells, it is essential to investigate MPK4-associated protein complex. Here we focus on identification of guard cell specific MPK4 complex using affinity purification following by mass spectrometry (AP-MS). Potential substrates were selected and validated with yeast two hybrid (Y2H).

2024-04-30 | PXD034347 | Pride

Stomatal opening under high temperatures is controlled by the OST1-regulated TOT3–AHA1 module

Project description:Plants continuously respond to changing environmental conditions to prevent damage and maintain optimal performance. To regulate gas exchange with the environment and to control abiotic stress relief, plants have pores in their leaf epidermis, called stomata. Multiple environmental signals affect the opening and closing of these stomata. High temperatures promote stomatal opening (to cool down), and drought induces stomatal closing (to prevent water loss). Coinciding stress conditions may evoke conflicting stomatal responses, but the cellular mechanisms to resolve these conflicts are unknown. Here we demonstrate that the high-temperature-associated kinase TARGET OF TEMPERATURE 3 directly controls the activity of plasma membrane H+-ATPases to induce stomatal opening. OPEN STOMATA 1, which regulates stomatal closure to prevent water loss during drought stress, directly inactivates TARGET OF TEMPERATURE 3 through phosphorylation. Taken together, this signalling axis harmonizes stomatal opening and closing under high temperatures and drought. In the context of global climate change, understanding how different stress signals converge on stomatal regulation allows the development of climate-change-ready crops.

2024-11-12 | PXD044300 | Pride

PAMP signal elicited-stomatal condensation of SAIR1 fine-tunes translation for immunity

Project description:Plant guard cells perceive pathogens and close stomata to prevent invasion. Biomolecular condensates are central to life growth and stress adaptation, while their roles in regulating stomatal immunity remain unclear. Here, we identify a guard cell-preferential RRM-type RNA-binding protein (RBP) named SAIR1 (stomatal immune RBP 1). Upon pathogen infection and the pathogen-associated molecular pattern flg22 signaling, SAIR1 undergoes phase separation and forms condensates in guard cells to mediate stomatal immunity. Upon flg22 signals, the activated kinases MPK3/6 phosphorylate SAIR1 to initiate its condensation in guard cells. SAIR1 condensates associate with critical defense mRNAs, including those involved in salicylic acid (SA) signaling. SAIR1 interacts with pivotal immune translation regulators such as PABPs and eIFiso4G, and enhances translation of defense mRNAs, ultimately promoting stomatal closure. Our findings reveal that SAIR1 phosphorylation, condensation, and interactions with mRNAs and translation regulators link flg22-MPK3/6 signaling with SA pathways to mediate stomatal immunity.

2025-07-07 | GSE286004 | GEO

Blue light and CO2 signals converge to regulate light-induced stomatal opening

Project description:Stomata regulate gas exchange between plants and the atmosphere by integrating opening and closing signals. Stomata open in response to low CO2 concentrations to maximize photosynthesis in the light; however, the mechanisms that coordinate photosynthesis and stomatal conductance have yet to be identified. Here, we characterize CBC1/2 [CONVERGENCE of BLUE LIGHT (BL) and CO2 1/2], two kinases that link BL, a major component of photosynthetically active radiation (PAR), and the signals from low concentrations of CO2 in guard cells. CBC1/CBC2 redundantly stimulate stomatal opening by negatively regulating S-type anion channels in response to both BL and low concentrations of CO2. CBC1/CBC2 function in the signaling pathways of phototropins and HT1 (HIGH LEAF TEMPERATURE 1). CBC1/CBC2 interacted with and were phosphorylated by HT1. We propose that CBCs regulate stomatal aperture by integrating signals from BL and CO2 and act as the convergence site for signals from PAR and low CO2.

2017-05-24 | MSV000081109 | MassIVE

Genes Involved in Meristemoid Cell Fate

Project description:The objective of this experiment is to identify genes that are regulated by the LRR-receptor-like protein TOO MANY MOUTHS (TMM) to control stomatal precursor (meristemoid) cell fate and behavior. An interesting aspect of the tmm phenotype is that mutant plants overproduce stomata on leaves, but lack stomata entirely on inflorescence stems and in other locations on the plant. Despite the failure to produce mature stomata, mutant stems produce presumptive stomatal precursors through asymmetric divisions. This suggests that normal TMM signaling is required to maintain meristemoid cell fate but not to execute formative asymmetric divisions. Because stem stomata develop in a morphogenetic wave from tip to base, it is possible to harvest tissues that contain meristemoids but not guard mother cells or mature stomata. This provides an opportunity to compare the gene expression profiles of tissues with meristemoids that maintain their cellular identity and ultimately produce stomata (Col gl1), to tissues in which meristemoids form but rapidly lose their cellular identity due to a failure in TMM signaling (tmm-1). Because guard mother cells and stomata are not present in the tissue harvested, genes that change in abundance during these developmental stages will be filtered out. Ultimately we hope to reveal genes that operate downstream of TMM to confer meristemoid cell fate or to provide 'niche factors' suitable for their development into stomata. For analysis, approximately 90 developing inflorescence stem tips were collected and flower buds and meristems removed prior to flash freezing for each replicate sample. The region harvested had been previously determined to contain developing meristemoids but not stomata or guard mother cells by microscopic observation. An identical region was harvested from tmm-1 mutant plants grown at the same time, in the same flat. 4 samples were used in this experiment.

2009-12-03 | E-GEOD-19256 | biostudies-arrayexpress

Chimeric repressors of GLKs confer tolerance to air pollutant by closing stomata in Arabidopsis

Project description:Regulation of stomatal movement is one of the effective strategies for developing resistant crops to air pollutant because stomata allows absorption of various air pollutants, such as ozone and sulfur dioxide. Transcription factor (TF) is a fascinating target of genetic manipulation for this end because TF regulates many genes simultaneously and methods of genetic manipulation are universally established. Here, we have screened transgenic Arabidopsis lines expressing chimeric repressor of TFs in high-concentration of ozone and found that the chimeric repressors of GOLDEN-LIKE1 (GLK1) and GLK2 (GLK1-SRDX and GLK2-SRDX) conferred strong tolerance to ozone. These 35S:GLK1/2-SRDX plants also showed sulfur dioxide tolerance. Their leaves showed lower rate of transpiration than wild type and a remarkable closed-stomata phenotype. The expression of the genes encoding K+ and water channels, including KAT1 and AKT1, which are involved in stomatal opening, was downregulated in 35S:GLK1-SRDX plants. Consistently, expression of GLK1-SRDX driven by the GC1 promoter, which has an activity only in guard cell, also induced closed-stomata and an ozone tolerant phenotype. On the contrary, 35S:GLK1/2 plants showed hypersensitivity to ozone and an opened-stomata phenotype. These data suggested that GLK1 and GLK2 have an ability to induce transcriptional change in guard cell and regulate stomatal movement. Our findings provide an effective tool to confer resistance to air pollutant by regulating stomatal aperture and improve crop productivity in future.

2015-10-01 | GSE42545 | GEO

Genes Involved in Meristemoid Cell Fate

2009-12-04 | GSE19256 | GEO