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Proteomic profiling and comparison of specific neurons in C. elegans

ABSTRACT: This work describes the results of using the MACSPI-SILAC method to profile and compare the proteome of dopaminergic neurons and touch receptor neurons in C. elegans.

INSTRUMENT(S):

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Qiao RAN

LAB HEAD: Chaogu ZHENG

PROVIDER: PXD070040 | Pride | 2026-03-30

REPOSITORIES: Pride

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			Action	DRS
	DArawdata.zip	Other
	DAvsTRNrawdata.zip	Other
	TRNrawdata.zip	Other
	checksum.txt	Txt
	results.zip	Other

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Publications

Integrating MACSPI and SILAC for Neuron Type-specific Proteomics in Caenorhabditis elegans.

Ran Qiao Q Huang Siyue S Li Xiang David XD Zheng Chaogu C

Molecular & cellular proteomics : MCP 20260228 4

Understanding neuronal differentiation and function requires precise proteomic characterization of distinct neuron types, yet existing methods face challenges in specificity and sensitivity. Here, we combine Methionine Analog-based Cell-Specific Proteomics and Interactomics (MACSPI) and SILAC to achieve neuron type-specific proteomic profiling in Caenorhabditis elegans. We demonstrate the utility of the methods by profiling and comparing the proteomes of two neuron types, namely the eight dopami ...[more]

PMID: 41765285

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