Dataset Information

Robotic Handling Preserves Induced Pluripotent Stem Cell Derived Vascular Smooth Muscle Cells Differentiation using a Weekend-free, Automatable, Low-variability, Low-cost (WALL) Protocol

ABSTRACT: As tissue engineering and high-throughput disease modeling develop further, necessitating large quantities of viable cells, automation will be essential. Robotic liquid handling systems are increasingly employed for generating large quantities of induced pluripotent stem cell (iPSC)-derived cells, which requires the critical development of scalable protocols. No studies have yet evaluated how using robotic liquid handling may alter the differentiation of cells compared to traditional manual handling. Here, we report a scalable differentiation protocol, which robustly generates iPSC-derived VSMCs (iVSMCs) from a cohort of female and male donors of varying age (N=5 lines). We demonstrate for the first time that these cells, differentiated through an automated robotic pipeline, had no significant differences in phenotype, as assessed morphologically and by proteomic analysis, compared to manual differentiation. The ability to automate this protocol facilitates the differentiation of iVSMC in large quantities, generating consistent, functionally relevant cells, setting a precedence for the application of automation in generating iPSC-derived cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Muhammad Alsherbiny