Proteomics

Dataset Information

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IP-MS Profiling of ANAPC1 and MP1 Interaction Networks in OVCAR3 Cells

ABSTRACT: We identified a pseudogene-derived chimeric transcript, vA-202, which encodes a partial ANAPC1-derived protein (MP1). To characterize its protein interaction network, we transiently expressed FLAG-tagged MP1 in OVCAR3 cells and performed immunoprecipitation using an anti-FLAG antibody. For comparison with the parental protein, endogenous ANAPC1 was immunoprecipitated using two antibodies: one specific to ANAPC1 alone and another recognizing an epitope shared with MP1. Corresponding IgG and peptide competition controls were included to eliminate nonspecific interactions. Immunoprecipitated protein complexes were subjected to LC-MS/MS analysis to identify interacting partners of both ANAPC1 and MP1.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Amruta Jadhav  

LAB HEAD: Dr. Sharmila A. Bapat

PROVIDER: PXD070186 | Pride | 2026-05-25

REPOSITORIES: Pride

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Dataset's files

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Action DRS
APC1_shared_IP_S1.csv Csv
APC1_shared_IP_S1_RAW.raw Raw
APC1_shared_IP_S2.csv Csv
APC1_shared_IP_S2_RAW.raw Raw
APC1_shared_IP_S3.csv Csv
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Publications

Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genome.

Jadhav Amruta R AR   Sahoo Sushmita S   Kuber Yash L YL   Hatwar Krutika K   Varanwal Avinash A   Tanwar Khushi K   Palwankar Shubhankar S   Chatrasal Gauri G   Mali Avinash M AM   Bapat Sharmila A SA  

Nucleic acids research 20260401 8

Genome evolution through rearrangements and selection drives the emergence of new genes, gene families, and species. Although pseudogenes (φgenes) are often regarded as "dead on arrival" due to disruptive mutations or loss of regulatory elements, our earlier work revealed that several φgenes remain transcriptionally active, challenging this assumption. Here, we show that segmental duplications at proximal loci can generate chimeric φgenes comprising contiguous sequences from two or more genes, o  ...[more]

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