Proteomics

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Application of VHH-immobilized cryogel-based immunoaffinity chromatography for isolation of extracellular vesicles from human plasma

ABSTRACT: Proteomics profiles of extracellular vesicles isolated using a new cryogel-based immunoaffinity chromatography system functionalized with single-domain VHH antibodies for selective isolation of EVs from human plasma.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Blood Plasma

SUBMITTER: Guillaume Médard  

LAB HEAD: Guillaume Médard

PROVIDER: PXD070239 | Pride | 2025-12-08

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20250521_EV.workflow Other
P2025-08_Core_IHTM_Jovana_cryogel_EVs_rep1_1_1200ng_Slot2-17_1_13471.d.zip Other
P2025-08_Core_IHTM_Jovana_cryogel_EVs_rep1_2_1200ng_Slot2-18_1_13440.d.zip Other
P2025-08_Core_IHTM_Jovana_cryogel_EVs_rep1_3_1200ng_Slot2-19_1_13445.d.zip Other
P2025-08_Core_IHTM_Jovana_cryogel_EVs_rep1_4_1200ng_Slot2-20_1_13450.d.zip Other
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Publications

Application of VHH-Immobilized Cryogel-Based Immunoaffinity Chromatography for Isolation of Extracellular Vesicles.

Terzić Jovana J   Filipović Lidija L   Mitić Ninoslav N   Stevanović Sanja S   Krstić Jugoslav J   de Marco Ario A   Courraud Julie J   Popović Milica M  

Molecules (Basel, Switzerland) 20251108 22

Extracellular vesicles (EVs) are nanosized structures involved in intercellular communication that have high potential as disease biomarkers and for the delivery of therapeutic cargos. However, translation to the clinic is hampered by time-consuming, low-yield, and poorly reproducible EV isolation methods. We describe a cryogel-based immunoaffinity chromatography system that exploits single-domain VHH antibodies as capture elements for the selective isolation of EVs from human plasma. Supermacro  ...[more]

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