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MclX modulates the process of mother cell lysis by SpoIIID in Bacillus thuringiensis

ABSTRACT: Cell differentiation is essential for the survival and adaptation of organisms. In Bacillus species, sporulation is a tightly coordinated program that culminates in mother cell lysis, a critical step for spore release. Despite its importance, the mechanisms modulating the process remain poorly understood. Here, we show that MclX regulates mother cell lysis by inhibiting SpoIIID, a sporulation-specific transcription factor that represses cwlC, which encodes a key hydrolase required for lysis. SpoIIID directly binds to the cwlC promoter, and its overexpression during sporulation markedly delays both the onset and progression of mother cell lysis. These findings support a model in which MclX modulates SpoIIID to control cwlC expression and lysis process. Collectively, this work defines a previously unrecognized MclX–SpoIIID–cwlC regulatory axis, providing new insight into the control of late sporulation events and the process of mother cell lysis.

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ORGANISM(S): Bacillus Thuringiensis

SUBMITTER: yanrong xu

LAB HEAD: Yanrong Xu

PROVIDER: PXD070258 | Pride | 2026-07-15

REPOSITORIES: Pride

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Project description:We previously observed reduced graft survival for kidney transplants having interstitial fibrosis with subclinical inflammation, but not fibrosis alone, on 1-year protocol biopsy. The current study aimed to determine whether fibrosis with inflammation at 1 year is associated with renal functional decline in a low-risk transplant cohort and to characterize the nature of the inflammation. Subjects were living-donor, tacrolimus/mycophenolate-treated transplant recipients without overt risk factors for reduced graft survival (n=151). Transplants with normal histology (n=86) or fibrosis alone (n=45) on 1-year protocol biopsy had stable renal function between 1 and 5 years, while those having fibrosis with inflammation (n=20) had declining glomerular filtration rate and reduced graft survival. Immunohistochemistry confirmed increased interstitial T-cells and macrophages/dendritic cells in the fibrosis with inflammation group. Gene expression was performed on a subset of biopsies in each group and demonstrated increased expression of transcripts related to innate and cognate immunity in transplants having fibrosis with inflammation. Pathway- and pathological process-specific analyses of microarray profiles revealed that, in fibrosis with inflammation, over-expressed transcripts were enriched for potentially damaging immunological activities including Toll-like receptor signaling, antigen presentation/dendritic cell maturation, interferon gamma-inducible response, cytotoxic T lymphocyte-associated and acute rejection-associated genes. Thus, fibrosis with inflammation in 1-year protocol biopsies is associated with reduced graft survival and function and with a rejection-like gene expression signature even in recipients with no clinical risk for inferior outcome. Early interventions aimed at altering rejection-like inflammation may favor improved long-term KTx survival. We analyzed gene expression from a group of 65 renal transplant recipients. Patient groups were carefully selected to include patients on the same immunosuppression (Prograf-MMF-Pred), transplant type (LD), absence of over post-transplant complications (AR, BK, DGF). For each patient a 1 year protocol biopsy was examined by conventional histology and gene expression. By histology the patients were categorized as histologically normal (n=25, i/cg/ci=0), IF/TA (n=24, i/cg=0, ci>0) and IFTA+i (n=16, cg=0, i/ci>0). This dataset is part of the TransQST collection.

			Action	DRS
	NKY0930.xlsx	Xlsx
	NKY0930_H1.raw	Raw
	NKY0930_H1_20211001183132.raw	Raw
	NKY0930_H1_20211001205730.raw	Raw
	NKY0930_H2.raw	Raw

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MclX modulates the process of mother cell lysis by SpoIIID in Bacillus thuringiensis

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