Dataset Information

Proteome profiling of rabies-infected and uninfected dog brain tissues, cerebrospinal fluids and serum samples

ABSTRACT: This study aimed to identify potential protein biomarkers for antemortem diagnosis of rabies in dogs, which are the principal reservoir hosts of the rabies virus. Lyssavirus rabies, the prototype species, effectively evades the host immune response allowing the infection to progress unnoticed until the onset of clinical signs. At this stage, the disease is irreversible and invariably fatal, with definitive diagnosis possible only post-mortem. Two hundred and thirty-one samples of brain tissue, cerebrospinal fluid, and serum were collected from apparently healthy dogs brought for slaughter for human consumption in South-East and North-Central Nigeria. All the brain tissue were subjected to a direct fluorescent antibody test to confirm the presence of lyssavirus antigen, and 8.7% (n = 20) were positive. Protein extraction and quantification was performed on 20 rabies-infected and -uninfected samples from each sample type, and only 10 rabies-infected and -uninfected samples were selected from each sample type as they had sufficient protein quantity for further sample preparation. Reduction and alkylation of the selected samples was performed and on-bead HILIC sample clean-up and tryptic digestion followed thereafter. The resulting peptides from each sample were injected into the EvoSep One LC system, coupled to the timsTOF HT mass spectrometer, using the standard dia-PASEF short gradient data acquisition method. Data was processed using Spectronaut (v19) and an unpaired t-test was performed to compare identified protein groups (proteins and their isoforms) between the rabies-infected and uninfected brain tissue, cerebrospinal fluid, and serum samples.

INSTRUMENT(S):

ORGANISM(S): Lyssavirus Rabies Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Brain, Cerebrospinal Fluid, Blood Serum

SUBMITTER: Ukamaka Eze

LAB HEAD: Rethabile Mokoena

PROVIDER: PXD070262 | Pride | 2026-01-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20250106_093332_20240601Ukamaka_BT_CSF_SR.sne	Other
	BT108N_S2-E3_1_4630.d.zip	Other
	BT113N_S2-E4_1_4671.d.zip	Other
	BT119P_S2-D11_1_4653.d.zip	Other
	BT137N_S2-E5_1_4667.d.zip	Other

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Publications

Proteome Profiling of Rabies-Infected and Uninfected Dog Brain Tissues, Cerebrospinal Fluids and Serum Samples.

Eze Ukamaka U UU Mokoena Rethabile R Ogbu Kenneth I KI Dubazana Sinegugu S Ngoepe Ernest C EC Munangatire Mparamoto M Ezeokonkwo Romanus C RC Anene Boniface M BM Buthelezi Sindisiwe G SG Sabeta Claude T CT

Proteomes 20251215 4

<h4>Background</h4>Rabies is among the oldest known zoonotic viral diseases and is caused by members of the <i>Lyssavirus</i> genus. The prototype species, <i>Lyssavirus rabies</i>, effectively evades the host immune response, allowing the infection to progress unnoticed until the onset of clinical signs. At this stage, the disease is irreversible and invariably fatal, with definitive diagnosis possible only post-mortem. Given the advances in modern proteomics, this study aimed to identify poten ...[more]

PMID: 41441337

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Project description:Shigui Ruan. Modeling the transmission dynamics and control of rabies in China. Mathematical Biosciences 286 (2017). Human rabies was first recorded in ancient China in about 556 BC and is still one of the major public-health problems in China. From 1950 to 2015, 130,494 human rabies cases were reported in Mainland China with an average of 1977 cases per year. It is estimated that 95% of these human rabies cases are due to dog bites. The purpose of this article is to provide a review about the models, results, and simulations that we have obtained recently on studying the transmission of rabies in China. We first construct a basic susceptible, exposed, infectious, and recovered (SEIR) type model for the spread of rabies virus among dogs and from dogs to humans and use the model to simulate the human rabies data in China from 1996 to 2010. Then we modify the basic model by including both domestic and stray dogs and apply the model to simulate the human rabies data from Guangdong Province, China. To study the seasonality of rabies, in Section 4 we further propose a SEIR model with periodic transmission rates and employ the model to simulate the monthly data of human rabies cases reported by the Chinese Ministry of Health from January 2004 to December 2010. To understand the spatial spread of rabies, in Section 5 we add diffusion to the dog population in the basic SEIR model to obtain a reaction-diffusion equation model and determine the minimum wave speed connecting the disease-free equilibrium to the endemic equilibrium. Finally, in order to investigate how the movement of dogs affects the geographically inter-provincial spread of rabies in Mainland China, in Section 6 we propose a multi-patch model to describe the transmission dynamics of rabies between dogs and humans and use the two-patch submodel to investigate the rabies virus clades lineages and to simulate the human rabies data from Guizhou and Guangxi, Hebei and Fujian, and Sichuan and Shaanxi, respectively. Some discussions are provided in Section 7.