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Microglial Adaptations to Chronic Nicotine in the Cerebellum: Proteomic Evidence for Neuroimmune Vulnerability

ABSTRACT: In this study, we investigated how chronic nicotine exposure reshapes microglial proteomes in the cerebellum using fluorescence-activated cell sorting (FACS) and mass spectrometry-based proteomics in adult male and female mice following 30 days of nicotine exposure.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Microglial Cell

SUBMITTER: Aya Nusir

LAB HEAD: Nadine Kabbani

PROVIDER: PXD070417 | Pride | 2026-06-08

REPOSITORIES: Pride

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			Action	DRS
	11072024_Nusir_FN_1.msf	Msf
	11072024_Nusir_FN_1.raw	Raw
	11072024_Nusir_FN_2.msf	Msf
	11072024_Nusir_FN_2.raw	Raw
	11072024_Nusir_FN_3.msf	Msf

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Microglial Adaptations to Chronic Nicotine in the Cerebellum: Proteomic Evidence for Neuroimmune Vulnerability.

Nusir Aya A Anthony Scott M SM Zhou Weidong W Kabbani Nadine N

Journal of proteome research 20260330 5

Smoking is a major public health concern with widespread effects on multiple organ systems, including the immune system. Chronic nicotine exposure can alter immune cell function through nicotinic receptors expressed on peripheral macrophages and microglia in the brain. Recent evidence indicates that the cerebellum is impacted by nicotine, contributing to motor and nonmotor outcomes, during drug use. In this study, we investigated the effect of chronic nicotine on microglia proteomes in the adult ...[more]

PMID: 41911483

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