Dataset Information

Unravelling the molecular responses of the yeast Schwanniomyces etchellsii to hyperosmotic stress in seawater medium using omic approaches. Experiment 1

ABSTRACT: Schwanniomyces etchellsii is an unconventional, halotolerant microorganism. Like some other yeasts, it can efficiently perform various biocatalytic transformations of organic compounds in seawater more effectively than in freshwater. In seawater, conversion rates are higher, by-product production is minimized, greater substrate loading is possible, and cells can be recycled for further use. To identify the molecular features that explain this behavior, comparative proteomic and lipidomic studies were conducted on cells grown in seawater and freshwater at various growth stages. The results showed higher expression of proteins involved in the stress response, such as glycerol-3-phosphate dehydrogenase, the glycerol transporter Stl1 and the P-type ATPase sodium pump Ena1, and several phospholipid biosynthesis proteins, including inositol-3-phosphate synthase and phosphatidate cytidylyltransferase, in seawater. Changes in metabolic enzymes and other proteins involved in responding to stimuli were also observed between the two conditions. Overall, cells grown in a freshwater medium exhibited higher levels of enzymes involved in biosynthetic processes. Differences in lipid profiles were also observed between cells grown in the two media. Higher levels of monoacyl and diacylglycerols were found in seawater, while higher levels of phospholipids containing serine and ethanolamine were found in freshwater. Consistent with more permeable membranes, cells grown in seawater exhibited lower levels of ergosterol.

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ORGANISM(S): Schwanniomyces Etchellsii

SUBMITTER: Luz Valero

LAB HEAD: Marcel·lí del Olmo

PROVIDER: PXD070465 | Pride | 2026-01-19

REPOSITORIES: Pride

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Unravelling the Molecular Responses of the Yeast <i>Schwanniomyces etchellsii</i> to Hyperosmotic Stress in Seawater Medium Using Omic Approaches.

Andreu Cecilia C Obis Èlia È Del Olmo Marcel Lí ML

International journal of molecular sciences 20251223 1

<i>Schwanniomyces etchellsii</i> is an unconventional, halotolerant microorganism. Like some other yeasts, it can efficiently perform various biocatalytic transformations of organic compounds in seawater more effectively than in freshwater. In seawater, conversion rates are higher, by-product production is minimized, greater substrate loading is possible, and cells can be recycled for further use. To identify the molecular features that explain this behavior, comparative proteomic and lipidomic ...[more]

PMID: 41516062

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Project description:Since the first human conceived through in vitro fertilisation in 1978, over 8 million babies have been born by assisted reproductive technologies (ART). Although most ART babies and children seem healthy, in recent years, several human and animal model studies have evidenced a potential impact of ART on long-term development. However, the long-term follow-up data in this field is still limited. Till now, studies are mainly focused on techniques such as in vitro fertilisation or in vitro culture, being the information from gametes/embryos cryopreservation field practically missing. Herein, we have developed an animal model to determine whether vitrified-thawed embryo transfer procedure has long-term consequences over the offspring. The birth weight, growth performance and adult body weight of the rabbits derived from vitrified-thawed embryos was compared with that of the naturally-conceived animals. In adulthood, the liver, heart, kidneys, spleen, lungs, gonads and adrenal glands of the males were weighed and compared. Moreover, some haematological and biochemical parameters were assessed on peripheral blood. Besides, some liver samples were obtained to perform a comparative proteomic study. The embryo vitrification-transfer process modified the birth weight and the growth pattern of the offspring, reducing the growth performance in a sex-specific manner. In adulthood, animals derived from vitrified embryos showed a significantly lower body, liver and heart weight. Molecular analyses revealed that vitrified-thawed embryo transfer procedure triggers concordant reprogramming of the liver proteome. The most relevant metabolic alteration denoted by the protein profile was that related to the oxidative phosphorylation, suggesting an impaired oxidative metabolism in the mitochondria. Furthermore, hints of dysregulation in the zinc and lipid metabolism were identified. These results evidence long-term consequences in the offspring derived from cryopreserved-transferred embryos and represented an evident example of the phenotypic plasticity exhibited by the mammalian embryo.

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	MO_191030_L3.wiff	Wiff

Dataset Information

Unravelling the molecular responses of the yeast Schwanniomyces etchellsii to hyperosmotic stress in seawater medium using omic approaches. Experiment 1

Dataset's files

Publications

Unravelling the Molecular Responses of the Yeast <i>Schwanniomyces etchellsii</i> to Hyperosmotic Stress in Seawater Medium Using Omic Approaches.

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Dataset Information

Unravelling the molecular responses of the yeast Schwanniomyces etchellsii to hyperosmotic stress in seawater medium using omic approaches. Experiment 1

Dataset's files

Publications

Unravelling the Molecular Responses of the Yeast &lt;i&gt;Schwanniomyces etchellsii&lt;/i&gt; to Hyperosmotic Stress in Seawater Medium Using Omic Approaches.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Unravelling the Molecular Responses of the Yeast <i>Schwanniomyces etchellsii</i> to Hyperosmotic Stress in Seawater Medium Using Omic Approaches.