Dataset Information

Human non-small cell lung cancer cell line NCI-H661 DPPA2-4 rapid immunoprecipitation mass spectrometry (RIME, LC-MSMS)

ABSTRACT: To investigate DPPA2/4 bound proteins on chromatin we performed rapid immunoprecipitation mass spectrometry (RIME) following double crosslinking of chromatin in NCI-H661 non-small cell lung cancer cells clonal cell lines (Wild Type clones c51 & c52, DPPA2 knockout clones c41 & c42, DPPA4 knockout clones c78 & c79 as well as DPPA2+4 double knockout clones c08 & c14).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Janith Seneviratne

LAB HEAD: Melanie Eckersley-Maslin

PROVIDER: PXD070627 | Pride | 2026-03-31

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	250814_JS-11489_1.raw	Raw
	250814_JS-11489_10.raw	Raw
	250814_JS-11489_10_uncalibrated.mzML	Mzml
	250814_JS-11489_11.raw	Raw
	250814_JS-11489_11_uncalibrated.mzML	Mzml

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Publications

Embryonic stem cell factors DPPA2/4 amplify active H3K4me3-H2AK119ub chromatin domains in non-small cell lung cancer.

Seneviratne Janith A JA Crisp Clare L CL Glancy Eleanor E Choy Natalie N Tan Winnie W Neve Matthew M Stammers Melanie M Wang Tongtong T Johnstone Ruby R Kaur Arshnoor A Fennell Katie A KA Burr Marian L ML Parker Benjamin L BL Shakeel Shabih S Eckersley-Maslin Melanie A MA

Genes & development 20260504 9-10

Embryonic regulators are often re-expressed in cancers; however, the functional and molecular significance of this is not always understood. The epigenetic priming factors developmental pluripotency-associated 2 and 4 (DPPA2/4) have crucial roles in early development and are implicated in cancer pathogenesis. We reveal that in non-small cell lung cancer (NSCLC), DPPA2/4 coexpression is associated with poorly differentiated tumors and impaired patient outcomes. Biochemically, human DPPA2/4 multim ...[more]

PMID: 41862192

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