Dataset Information

High-resolution spatial proteomics of Plasmodium falciparum schizonts using hyperLOPIT and TMT-based mass spectrometry

ABSTRACT: This dataset contains a hyperLOPIT spatial proteomics experiment performed on Plasmodium falciparum to determine the subcellular localization of parasite proteins. Schizont-stage parasites were fractionated by density gradient centrifugation, and collected fractions were labelled with tandem mass tags (TMT) for multiplexed quantification. Peptides were analysed by LC–MS/MS on an Orbitrap Fusion Lumos mass spectrometer. Protein identification and quantification were performed using Proteome Discoverer with the Sequest HT search engine against the P. falciparum reference proteome and human proteome. The dataset supports high-resolution mapping of organelle localization and contributes to understanding the compartmental organization of the malaria parasite proteome.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Plasmodium Falciparum (isolate 3d7)

TISSUE(S): Blood Cell, Blood

DISEASE(S): Malaria

SUBMITTER: Scott Chisholm

LAB HEAD: Ross Waller

PROVIDER: PXD070842 | Pride | 2026-06-01

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20-01-2024_hao_group_universal_contaminants_ds.fasta	Fasta
	20-01-2024_swissprot_reviewed_human_proteome_noisoforms.fasta	Fasta
	PFA1.raw	Raw
	PFA10.raw	Raw
	PFA11.raw	Raw

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Publications

The spatial proteome of the Plasmodium falciparum schizont illuminates the composition and evolutionary trajectories of its organelles.

Chisholm Scott A SA Flores Victor V Kemp Alison A Breckels Lisa M LM Koreny Ludek L Dos Santos Pacheco Nicolas N Barylyuk Konstantin K Kuroshchenkova Anna A Lilley Kathryn S KS Rayner Julian C JC Waller Ross F RF

Nature communications 20260530

Malaria is caused by apicomplexan parasites of the genus Plasmodium, with all malaria symptoms and pathology caused by parasite stages that develop within, or transit between, host erythrocytes. The ability of Plasmodium cells to parasitise erythrocytes depends on distinctive intracellular compartments associated with invasion, as well as the development of unique cellular niches within the infected host cell. However, our understanding of the biology of the malaria parasite is limited by the fa ...[more]

PMID: 42218142

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