Dataset Information

Simulated microgravity-induced changes in SUMOylation and protein expression in Saccharomyces cerevisiae

ABSTRACT: This study investigated the impact of simulated microgravity (SMG) on SUMOylation and protein expression in Saccharomyces cerevisiae. Utilizing a rotating wall vessel to simulate microgravity conditions, the research employed SILAC labeling and bottom-up proteomics to differentiate gravity vs SMG for SUMOylation (exp 1742) and for protein expression (exp 1772). N=6 for each experiment.

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ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Steven Hartson

LAB HEAD: Jeremy A. Sabo

PROVIDER: PXD070877 | Pride | 2026-01-19

REPOSITORIES: Pride

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Simulated Microgravity-Induced Changes in SUMOylation and Protein Expression in <i>Saccharomyces cerevisiae</i>.

Sabo Jeremy A JA Hartson Steven D SD

International journal of molecular sciences 20251219 1

Microgravity during space travel induces significant regulatory changes in the body, posing health risks for astronauts, including alterations in cell morphology and cytoskeletal integrity. The Small Ubiquitin-like Modifier (SUMO) is crucial for cellular adaptation, regulating DNA repair, cytoskeletal dynamics, cell division, and protein turnover-all processes affected by microgravity. To determine the extent to which SUMO mediates the cellular response to microgravity stress, <i>Saccharomyces c ...[more]

PMID: 41515919

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Project description:Gravitational biology and its effects on cancer cells are of great interest, especially today that space is more accessible than ever. Despite advances in space research, little is known about the impact of microgravity on tumor and its biology and data from the literature are often contradictory due to different setup, experimental designs and analyzed time-points. Exploiting the Random Positioning machine we evaluated on pancreatic cancer cells the effects of long-term exposure to simulated microgravity (SMG) performing a large proteomic, lipidomic and transcriptional analysis at 1, 7 and 9 days. Results indicate that SMG affects cellular morphology through a time-dependent activation of “Regulation of actin-based motility by Rho”and “Cdc42” pathways that contribute to the dynamic actin rearrangement involved in the formation of 3D spheroidal structures and in the enhancement of epithelial-to mesenchymal transition. To support energy stress remediation and counteract massive apoptosis activation and cell proliferation inhibition, induced by SMG, tumor cells downregulate the protein translational machinery, and undergo a metabolic reprogramming orchestrated by the activation of HIF-1a and PI3K/Akt pathways. These two pathways pave the way for glucose metabolism encouraging upregulation of glycolysis, mitochondrial activity, Tri-carboxylic acid cycle and fatty acid synthesis (rather than B oxidation), suggesting a de novo synthesis of triglycerides involved in the formation of membrane lipid bilayer and signaling mediators. This metabolic plasticity, typical of cancer stem cells, is in accordance with the SMG-mediated activation of PI3K/Akt/NFkB/ERK5/IL-8 signaling axis that significantly upregulates the expression of stem-associated proteins and expansion of cancer stem cells with a more mesenchymal phenotype and improved migratory capability. Altogether, our findings clearly indicate that microgravity induces cancer cell transformation towards the acquisition of cancer stem cell-like features, with a more aggressive and metastatic potential and pave the base for future study of response to anti neoplastic drugs under simulated microgravity.

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Dataset Information

Simulated microgravity-induced changes in SUMOylation and protein expression in Saccharomyces cerevisiae

Dataset's files

Publications

Simulated Microgravity-Induced Changes in SUMOylation and Protein Expression in <i>Saccharomyces cerevisiae</i>.

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Dataset Information

Simulated microgravity-induced changes in SUMOylation and protein expression in Saccharomyces cerevisiae

Dataset's files

Publications

Simulated Microgravity-Induced Changes in SUMOylation and Protein Expression in &lt;i&gt;Saccharomyces cerevisiae&lt;/i&gt;.

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OmicsDI is part of the ELIXIR infrastructure

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Simulated Microgravity-Induced Changes in SUMOylation and Protein Expression in <i>Saccharomyces cerevisiae</i>.