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Proteomics analysis of Control and Palbociclib-treated HeLa cells

ABSTRACT: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to profile the proteomes of Control and Palbociclib-treated HeLa cells. For protein identification and quantification, mass spectrometry data from fractionated samples (data-dependent acquisition, DDA; 6 fractions) and single-shot samples (data-independent acquisition, DIA) were utilized to construct a DDA library and a direct-DIA library, respectively. These libraries were computationally merged into a hybrid spectral library using Spectronaut software (Biognosys). Subsequent functional annotation was performed through Gene Ontology (GO) and InterPro (IPR) analyses using the InterProScan-5 tool against the non-redundant protein database. Additionally, protein family and pathway analyses were carried out using the Clusters of Orthologous Groups (COG) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Yihang Wang

LAB HEAD: Lijun Liu

PROVIDER: PXD070997 | Pride | 2025-12-04

REPOSITORIES: Pride

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Project description:Diabetic wounds require continuous and coordinated modulation of the microenvironment concurrent with tissue regeneration, yet this remains a significant challenge. As a proof of concept, we herein propose to use dimeric copper peptide for diabetic wound treatment. The dimeric copper peptide (D-CuP) was first synthesized and then incorporated into a reactive oxygen species (ROS)-responsive hydrogel matrix to improve therapeutic compliance, culminating in the formulation of G/D-CuP. Compared to monomer copper peptide (CuP), a wound healing agent, D-CuP exhibits multivalency, enhanced biologic stability against proteases and the broad biological activities, such as anti-inflammatory and antioxidative properties, while promoting angiogenesis and fibroblast proliferation and migration. Meanwhile, the hydrogel matrix, exhibiting excellent ROS-scavenging capabilities, has been engineered to an intelligent drug reservoir for wound-responsive release of D-CuP at the wound site while simultaneously attenuating inflammatory responses. Ultimately, the G/D-CuP group demonstrated superior therapeutic efficacy, achieving in 97.2% closure of infected wounds.In this study, we present a proof-of-concept study to optimize the treatment of diabetic wounds. This method integrates a dimeric copper peptide hydrogel (G/D-CuP) to address several critical factors: i) The design and synthesis of the dimeric copper peptide (D-CuP) feature a broad range of targets and enhanced biological activities, such as anti-inflammatory and antioxidative properties, while promoting angiogenesis and fibroblast proliferation and migration; ii) Utilizing the steric hindrance conferred by dimerization reduces protease access to the active site and significantly improving stability against proteases; iii) A ROS-responsive hydrogel matrix has been synthesized, exhibiting superior ROS-scavenging capabilities, functioning as an intelligent drug reservoir that enables the controlled release of D-CuP at the wound interface while simultaneously attenuating inflammatory responses in the microenvironment; iv) G/D-CuP possesses outstanding deformability and spreadability, allowing it to adapt to any wound shape; v) The synthesis and preparation process of G/D-CuP are straightforward and cost-effective, making it highly promising for clinical translation. This multifunctional treatment platform is anticipated to accommodate the complex and dynamic biological processes involved in diabetic wound healing, thereby significantly improving the overall efficacy of wound healing treatments.

			Action	DRS
	Palbociclib_pr-1.raw	Raw
	Palbociclib_pr-2.raw	Raw
	Palbociclib_pr-3.raw	Raw
	checksum.txt	Txt
	control_pr-1.raw	Raw

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Proteomics analysis of Control and Palbociclib-treated HeLa cells

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