Proteomics

Dataset Information

LC-MS/MS of co-immunoprecipitation of 3xFLAG-UFM1 from ribosomal pellet in control and upon anisomycin treatment

ABSTRACT: Clearance of arrested nascent polypeptides resulting from ribosomal stalling is essential for proteostasis. Stalled endoplasmic reticulum (ER)-bound ribosomes are marked by ubiquitin-fold modifier 1 (UFM1) on large ribosomal subunit protein RPL26, but the precise role of this process in ribosome-associated quality control (RQC) remains poorly understood. To identify proteins associated with UFMylated ribosomes, we isolated ribosomes from HCT116 cells expressing 3xFLAG-UFM1 upon stalling induction with anisomycin and in control conditions and preformed immunoprecipitation of 3xFLAG-UFM1.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colorectal Cancer Cell Line

SUBMITTER: Richard Imre  

LAB HEAD: Guelsuen Elif Karagoez

PROVIDER: PXD071028 | Pride | 2026-03-23

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20251003_07_A3_A2_Grujic_Dagdas_GMI_ID2317_2315_human_fullDB_UFM_Ratios.pdResult Other
20251003_A3_N8_Grujic_Dagdas_GMI_ID2317_S01_Lysate-control-1.raw Raw
20251003_A3_N8_Grujic_Dagdas_GMI_ID2317_S02_Lysate-control-2.raw Raw
20251003_A3_N8_Grujic_Dagdas_GMI_ID2317_S03_Lysate-control-3.raw Raw
20251003_A3_N8_Grujic_Dagdas_GMI_ID2317_S04_Lysate-ANS-1.raw Raw
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