Dataset Information

Hormone-dependent secretome profiling of bovine oviductal epithelial organoids

ABSTRACT: This project characterizes the hormone-dependent secretome of bovine oviductal epithelial organoids. Organoids were stimulated with estradiol or progesterone to mimic follicular and luteal phases, and their secretions were analyzed by LC-MS/MS. The resulting proteomic profiles closely matched native oviductal fluid and revealed clear endocrine-specific signatures, demonstrating that organoids faithfully reproduce the molecular and secretory landscape of the bovine oviduct.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Oviductal Fluid, Oviduct

SUBMITTER: Sergio Navarro

LAB HEAD: Vicente Perez-Garcia

PROVIDER: PXD071140 | Pride | 2026-06-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DP_ov_19_01_4105.d.tar.zip	Other
	DP_ov_19_02_4110.d.tar.zip	Other
	DP_ov_19_06_4113.d.tar.zip	Other
	DP_ov_39_01_4112.d.tar.zip	Other
	DP_ov_39_02_4108.d.tar.zip	Other

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Publications

Hormonally responsive bovine oviductal organoids recapitulate native oviductal secretions and enhance sperm capacitation.

Navarro-Serna Sergio S Romero-Aguirregomezcorta Jon J Hernández-Díaz Nathaly N Ferrero-Micó Ana A Coy Pilar P Pérez-García Vicente V

Cellular and molecular life sciences : CMLS 20260421 1

The oviduct provides the dynamic microenvironment that supports fertilization and early embryo development yet replicating its hormonally regulated secretory activity in vitro remains a major challenge. Here, we established bovine oviductal epithelial organoids that exhibit a polarized epithelial organization and endocrine responsiveness, while recapitulating key features of the native oviduct. Exposure to either estradiol or progesterone resulted in distinct transcriptomic and proteomic landsca ...[more]

PMID: 42009932

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Project description:Purpose: Transcriptome profiling of organoids/tissues derived from murine oviduct and OSE was performed to study and compare the gene expression profiles in the both suspected origins of HG-SOC. CRISPR-Cas9 modified oviductal organoids (TBP clones, which were mutated in Trp53, Brca1 and Pten), the tumors derived upon xenotransplantation of the TBP-clones and subsequent tumor-derived organoids were additionally profiled to show the transcriptional change upon aquiring mutations and tumor development. Methods: Both oviductal and OSE organoids as well as oviductal TBP-clones and tumor-derived organoids were grown in Wnt-conditioned media prior harvesting for RNA extraction. Additionally, RNA was extracted from healthy oviductal and OSE tissues and Ovi-TBP-clone-derived tumor tissues. RNA was converted to cDNA and libraries were prepared using the CelSeq2 method and sequenced. Samples were sequenced on Illumina NextSeq500 by using 75-bp paired-end sequencing. Paired-end reads from Illumina sequencing were aligned to the mouse genome (GRCm38 assembly) by BWA. DESeq2 (v1.18.0) package was used for read normalization and differential expression analysis. Gene set enrichment analysis (GSEA) was performed using gene lists for motile cilium assembly against normalized RNA-seq reads of healthy oviductal an OSE organoids using GSEA software v3.0 beta2. GSEA was additionally used to detect enrichment of signature gene sets of different HG-SOC subtypes in the TBP-clone-derived murine tumors Results: Transcriptome analysis of oviductal and OSE organoids/tissues revealed tissue-specific gene expression pattern in the organoid systems. In contrast to OSE counterparts, oviductal organoids showed enrichment in motile cilium assembly genes, reflecting the presence of ciliated cells in this model. Results: Oviductal TBP-clones gave rise to different tumor phenotypes with distinct gene expression profiles. Analysis of the transcriptomes of these tumors divided the TBP-clone-derived tumors into two distinct HG-SOC molecular subtypes (differentiated- or immunoreactive-like subtypes), confirming the potential of organoids in modeling HG-SOC. Results: The tumor-derived organoids retained the distinct phenotypes observed in the originating Ovi-TBP-clone derived tumors. Subset of tumors showed epithelial and others mesenchymal features that were maintained in the respective organoid cultures also in the gene expression level. Conclusions: RNA sequencing showed tissue specific differences in murine oviductal and OSE organoids, and revealed HG-SOC-like features of oviductal mutants.